摘要
对宁夏2012～2016年急性驰缓性麻痹(Acute flaccid paralysis,AFP)病例监测中分离到的非脊灰肠道病毒(Non-polio enterovirus,NPEV)进行血清型别鉴定和基因特征分析。采用RD和L20B两种细胞对采集自AFP病例及其接触者的粪便标本进行病毒分离,分离获得的毒株使用肠道病毒简并引物进行全长VP1区基因扩增、核苷酸序列测定、基因型别鉴定和基因系统发生学分析。2012～2016年共收集959份粪便标本,其中采自AFP病例粪便标本426份,密接者粪便标本533份,分离到67株NPEV,除4株未鉴定出型别外,共鉴定出23个肠道病毒血清型,其中A组肠道病毒(EV-A)23株(36.51%),包括7个血清型,以CV-A4(12.69%)、CV-A8(6.35%)和EV-A71(6.35%)为主;B组人肠道病毒(EV-B)40株(63.5%),包括17个血清型,以CV-B组4、2和3为主,分别占11.11%、9.52%和7.93.%,未鉴定出C组和D组人肠道病毒。宁夏2012～2016年从AFP病例及密接者标本中分离到的NPEV以EV-B组为主要型别,占63.49%,随着时间的推移一些主要流行的血清型别发生了变化。
To identify the serotype and phylogenie analysis of non-polio enterovirus(NPEV)from the acute flaccid paralysis(AFP)cases in Ningxia,China during 2012～2016.RD cell and L20 Bcell were inoculated for virus isolation.The complete nucleotide sequencing of the VP1 region were amplified by RT-PCR with degenerate primers and sequenced,sequence were compared with the database of GenBank by the BLAST algorithm to identify the serotype and analyzed the evolution by constructing phylogenetic tree.67 strains were isolated from stool specimens of 426 AFP cases and 533 contacts,According to the result of the molecular identification,23 strains belonged to 7 serotypes of EV-A(36.51%),the CV-A4(12.69%),CV-A8(6.35%)and EV-A71(6.35%)were the main serotypes;40 trains belonged to 17 serotypes of EV-B(63.5%),the CV-B4(36.5%),CV-B2(36.5%)and CV-B3(36.51%)were the main serotypes.No strain belonged to EV-C and EV-D species.HEV-B was the most predominant serotype of NPEV in Ningxia AFP surveillance during 2012-2016.The main serotypes transformed with the change of time.
出处
《病毒学报》
CAS
CSCD
北大核心
2018年第6期884-888,共5页
Chinese Journal of Virology
