期刊文献+

多吡啶铜(Ⅱ)配合物的合成、表征与DNA作用及抗肿瘤活性 被引量:5

Synthesis,Characterizes,Interaction with DNA and Antiproliferative Activity of Cancer Cells by Polypyridyl Copper(Ⅱ) Complex
下载PDF
导出
摘要 合成了一种多吡啶铜(Ⅱ)配合物,用红外光谱、元素分析、X-射线单晶衍射法对配合物的组成和结构进行了表征。该配合物的晶体属单斜晶系,P2(1)/c空间群。用荧光光谱、紫外光谱研究了配合物与小牛胸腺DNA的键合作用。结果表明,配合物与DNA的键合作用属部分插入,结合常数K_b=8.0×104L/mol。用凝胶电泳法研究了配合物对p BR322DNA的切割作用。实验表明,配合物用310 nm光辐射15 min,可将p BR322 DNA断裂为开口缺刻型和线型DNA。采用MTT法研究了配合物对人乳腺癌细胞和肺癌细胞的体外抗肿瘤活性。结果发现,所合成配合物能有效抑制这两种细胞增殖。 A tridentate polypyridyl Cu( Ⅱ) complex has been synthesized and confirmed by IR,elemental analysis,and X-ray single crystal diffraction. The crystal structure of complex belongs to monoclinic system with space group P2( 1) / c. Interactions of the complex with calf thymus DNA binding have been investigated by fluorescence spectroscopy,absorption spectroscopy. The spectrophotometric results showed that the mode of binding could be partial intercalation and its binding constant Kb is 8. 0 × 104 L / mol.The agarose gel electrophoresis showed complex can cleave circular plasmid pBR322 DNA to both nicked and linear forms when it was irradiated at 310 nm for 15 min. In vitro cytotoxic activities of the complex against human breast carcinoma( MCF-7) and human lung carcinoma( A549) were investigated by MTT assay method. The results indicated that complex can effectively inhibit the proliferation.
出处 《化学试剂》 CAS 北大核心 2015年第12期1080-1084,1090,共6页 Chemical Reagents
基金 河南省科技厅基础与前沿技术研究资助项目(112300410189)
关键词 多吡啶配体 铜(Ⅱ)配合物 晶体结构 DNA 键合 断裂 抗肿瘤活性 polypyridine ligand copper(Ⅱ) complex crystal structure DNA binding cleavage antiproliferative activity
  • 相关文献

参考文献19

  • 1ROSENBERG B, VANCAMP L, KRIGAS T. Platinum compounds : a new class of potent antitumour agents [J]. Nature, 1969,222 ( 4 ) : 385-386.
  • 2GASSER G, METZLER-NOLTE N. The potential of orga- nometallic complexes in medicinal chemistry [J]. Curr. Opin. Chem. Biol. ,2012,16(2) :84-91.
  • 3MARZANO C ,PELLEI M ,TISATO F,et al. Copper com- plexes as anticancer agents [J]. Anticancer Agents Med. Chem. ,2009,9(2) : 185-211.
  • 4MESSORI L,ABBATE F,MARCON G,et al. Gold( Ⅲ ) complexes as potential antitumor agents:solution chemis- try and cytotoxic properties of some selected gold ( Ⅲ ) compounds[J]. J. Med. Chem. , 2000,43 ( 19 ) : 3 541- 3 548.
  • 5SUNTHARALINGAM K, MENDOZA O, DUARTE A A, et al. A platinum eomplex that binds 'non-covalently to DNA and induces cell death via a different mechanism than eisplatin [J]. Metallomics ,2013,5:514-523.
  • 6BARTON J K, DANNENBERG J J, RAPHAEL A L. En- antiomeric selectivity in binding tris (phenanthrolinc) zinc( II ) to DNA[J]. J. Am. Chem. Soc.,1982,104 (18) :4 967-4 969.
  • 7SITLANI A S, LONG E C, PYLE A M, et al. DNA photo- cleavage by phenanthrenequinone diimine complexes of rhodium ( RI ) : shape-selective recognition and reaction [J]. J. Am. Chem. Soc. ,1992,114(7) :2 303-2 312.
  • 8GUNTER T E,GAVIN C E,GUNTER K K. The case tor manganese inter-action with m itochondria [J]. Neurotoxi- cology, 2009,30 ( 4 ) :727-729.
  • 9黄雪英,孟祥高,张妍,王莉,刘长林.多苯并咪唑锰(Ⅱ)配合物的合成及对DNA凝聚的促进作用[J].高等学校化学学报,2012,33(6):1151-1157. 被引量:10
  • 10CHASTON T B, WATTS R N, YUAN Jun, et al. Potent antitumor activity of novel iron chelators derived from di- 2-pyridylketone isonicotinoyl hydrazone involves fenton- derived free radical gen-eration [J]. Clin. Cancer Res. , 2004,10(21) :7 365-7 374.

二级参考文献74

共引文献23

同被引文献36

引证文献5

二级引证文献4

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部