摘要
目的在细胞水平研究PARP-2选择性抑制剂对心肌肥大的调控作用。方法新生SD大鼠心肌细胞原代培养,然后使用PARP-2选择性抑制剂UPF1069来处理细胞后,通过检测心肌细胞表面积及肥大基因ANF、BNP和β-MHC的mRNA表达变化来作为评判心肌细胞肥大的指标。结果使用PARP-2选择性抑制剂UPF1069沉默PARP-2能够逆转AngⅡ所诱导心肌细胞的肥大。结论选择性的沉默PARP-2能够抑制AngⅡ诱导的心肌细胞肥大。
Objective： To explore the effects of PARP-2 on the cardiac hypertrophy in vitro. Methods： The expression of PARP-2was knocked down by using selective inhibitor UPF1069 in neonatal rat cardiomyocytes and the cardiomyocyte hypertrophy was evaluated by measuring the mRNA levels of ANF,BNP,and β-MHC and cell-surface area. Results：In the neonatal rat cardiomyocytes,the expression of PARP-2 knocked down by using selective inhibitor UPF1069,indicating that endogenous PARP-2 played a positive regulatory role in cardiac hypertrophy. Conclusion： Knockdown of PARP-2 protects cardiomyocytes from hypertrophy.
出处
《泰山医学院学报》
CAS
2015年第10期1081-1084,共4页
Journal of Taishan Medical College
基金
国家自然科学基金资助项目(No.81072641
No.81273499)
