摘要
目的研究冰岛刺参岩藻聚糖硫酸酯(fucoidan from the sea cucumber Cucumaria frondosa,Cf-FUC)对胰岛素抵抗小鼠炎症反应的改善作用。方法以高脂高糖饲料饲喂法建立胰岛素抵抗小鼠模型。雄性C57BL/6J小鼠随机分为正常对照组、模型对照组、阳性对照组、Cf-FUC组。正常对照组饲喂标准饲料,其它组饲喂高脂饲料。阳性对照组、Cf-FUC组分别在饲料中添加罗格列酮(rosiglitazone,RSG,1mg·kg^(-1)·d^(-1))、Cf-FUC(80mg·kg^(-1)·d^(-1))。各组小鼠自由摄食摄水19周。实验结束后,检测血清肿瘤坏死因子α(TNF-α)、MIP-1、IL-1β、IL-6和IL-10表达水平,荧光定量PCR方法检测小鼠脂肪组织炎症细胞因子基因mRNA表达水平,Western方法检测JNK1、IKKβ磷酸化蛋白水平及细胞核和细胞质中NFκB蛋白表达量。结果 Cf-FUC可显著降低胰岛素抵抗小鼠血清TNF-α、MIP-1、IL-1β和IL-6浓度,增加IL-10浓度;抑制小鼠脂肪组织TNF-α、MIP-1、IL-1β和IL-6基因mRNA表达,促进IL-10基因mRNA表达;抑制JNK1和IKKβ的磷酸化,增加细胞质中NFκB蛋白表达量,减少细胞核中NFκB蛋白表达量。结论Cf-FUC能通过抑制JNK和IKKβ/NFκB信号通路抑制促炎症因子分泌,增加抑炎症因子分泌,改善胰岛素抵抗小鼠的炎症反应。
Objective To unravel the effects of fucoidan from the sea cucumber Cucumaria frondosa（CfFUC）on ameliorating inflammatory response in insulin resistant mice.Methods The insulin resistant model mice were established by feeding a diet of high-fat and high-sucrose.Male C57BL/6Jmice were randomly assigned into the normal control group,the model control group,the positive control group,and Cf-FUC group.The mice in the normal control group were fed the standard diet,and the animals in the other groups were fed the high-fat diet.Rosiglitazone（RSG,1mg·kg^-1·d^-1）and Cf-FUC（80mg·kg^-1·d^-1）were added into the diets of the positive control and Cf-FUC groups,respectively.All animals were fed with diet and water freely for 19 weeks.After experiments,serum tumor necrosis factor-α（TNF-α）,MIP-1,IL-1β,IL-6,and IL-10 levels were determined.The mRNA expression of inflammatory cytokines genes in adipose tissues was detected using quantitative real-time PCR.The phosphorylated protein expression of JNK1 and IKKβ,as well as NFκB protein expression in cell nucleus and cytoplasm were assayed using Western blotting.Results Cf-FUC decreased serum TNF-α,MIP-1,IL-1β,and IL-6concentrations significantly and increased IL-10 level of insulin resistant mice.CfFUC significantly inhibited TNF-α,MIP-1,IL-1β,and IL-6genes mRNA expression,and promoted IL-10 gene mRNA expression in adipose tissues.Cf-FUC inhibited JNK1 and IKKβphosphorylation,increased NFκB protein expression in cytoplasm,and reduced NFκB protein expression in cell nucleus.Conclusion Cf-FUC significantly inhibited the secretion of pro-inflammatory cytokines,increased the secretion of anti-inflammatory cytokines,and alleviated inflammatory response via inhibiting JNK and IKKβ/NFκB signal channel of insulin resistant mice.
出处
《中国海洋药物》
CAS
CSCD
2015年第6期49-56,共8页
Chinese Journal of Marine Drugs
基金
国家自然科学基金项目(31371876
31471648)
浙江海洋学院科研启动经费资助
