摘要
采用SDS-PAGE电泳分析褐飞虱成虫和各龄期若虫的可溶性蛋白,结果表明,成虫各类样品中,条带数量基本相同,只发现一条雌性成虫所特有的蛋白条带,而多数条带在不同翅型或性别样品中存在着含量差异。如有4条条带在长翅型中的含量明显高于短翅型中的含量;在雌、雄成虫样品的比较中则发现2条条带在雄虫中含量高于雌虫,而另有3条条带在雌虫中含量高于雄虫。若虫样品随着龄期升高,条带逐渐增多,发现有4条条带在各个龄期中均有表达,1条条带只在5龄若虫样品中出现,另有1条条带从4龄期开始出现。此外,还发现有2条条带在长翅芽若虫样品中的含量(9.029,9.563 ng/μL)明显高于短翅芽若虫样品中的含量(5.133,3.111 ng/μL)。并讨论了这些差异条带与翅型或性别分化方面的关系。
The soluble proteins in Nilaparvata lugens adults and nymphs were analyzed by SDS-PAGE electrophoresis. Results showed that there were almost equal number protein bands in all types of adult samples,most bands in different wing or gender samples existed content differences except female adultspecific protein band. There were four bands significantly higher in long-winged adult samples than in short-winged adult samples; in the comparison of male and female adult samples,it was found that there were two bands higher in males than in females,while other three bands higher in females than in males.The bands in nymph samples were gradually increased with the increase of age,four bands were constantly expressed in each instar period,one more band appeared in 5^th instar nymph samples,and one band could be detected from the 4^th instar. In addition,the contents of two bands were found much higher in long wing bud nymphs（ 9. 029,9. 563 ng / μL） than those in short wing bud nymphs（ 5. 133,3. 111 ng /μL）. Last we discussed the relationships among the different bands and wing dimorphism or sex differentiation in insects.
出处
《中山大学学报（自然科学版）》
CAS
CSCD
北大核心
2015年第6期27-30,共4页
Acta Scientiarum Naturalium Universitatis Sunyatseni
基金
国家自然科学基金资助项目(311771844)
广东省自然科学基金资助项目(2015A030313114)
