摘要
2014年春季,在湖北省武汉市发现种植的番茄表现植株矮缩,叶片上卷,叶缘黄化等症状。PCR检测结果显示,所有采集的病样中均存在菜豆金色花叶病毒属病毒。进一步通过滚环扩增方法获得了该病毒的湖北分离物HB01的全基因组。基因克隆及序列分析结果表明,该病毒基因组全长为2 781nt,与已报道的番茄黄化曲叶病毒(TYLCV)各分离物同源性在89.0%以上,而与来自中国不同地区的TYLCV分离物的同源率均在97.0%以上。因此,HB01属于TYLCV的一个分离物。
In spring of 2014,tomato plants in Hubei Province exhibited severely dwarf,and leaves upward curling and prominent yellowing along margins symptoms.PCR detection indicated that the diseased plants were infected by Begomovirus .Using rolling circle amplification （RCA）method,the full-length DNA of the Begomovirus iso-late HB01 was obtained.Sequence analysis and BLAST results indicated that the genome of HB01 had 2 781 nucle-otides,which had 89.0% identities with other isolates of Tomato yellow leaf curl virus from GenBank,and more than 97.0% identities with other isolates of Tomato yellow leaf curl virus from China.Therefore,the virus infec-ted tomato in Hubei was an isolate of Tomato yellow leaf curl virus .
出处
《植物保护》
CAS
CSCD
北大核心
2015年第5期233-236,共4页
Plant Protection
基金
公益性行业(农业)科研专项(201003065)
广东省科技计划项目(2012A020200015)
广东省农业科学院院长基金(201424)
关键词
番茄黄化曲叶病毒
基因克隆
序列分析
Tomato yellow leaf curl virus
gene cloning
sequence analysis
