摘要
发酵罐装填系数0.6,控制罐压0.08 Mpa,采用单因素试验方法考察搅拌转速、种子液OD600、接种量和通气量4个因素对壳聚糖酶发酵的影响。根据试验结果确定该菌种10 L发酵罐发酵条件：搅拌转速250 r/min,种子液OD600=0.8~1.0,通气量2.0 vvm,接种量3%,其发酵周期56 h,酶活力在52 h达到最大,为（35.2±0.5）U/mL。补料工艺发酵周期为64 h,酶活力在60 h达到最大,为（46.4±0.5）U/mL。补料工艺能维持产酶期菌丝形态和延长产酶时间,从而提高产酶,最终补料工艺比批次发酵产酶提高了32%。该研究为壳聚糖酶工业化生产奠定了基础。
Effects of agitator speed, aeration rate, inoculation amount and inoculum age on chitosanase production were studied by single factor experiments designs under the conditions of 0.6 filling coefficient and 0.08 Mpa tank pressure. The results showed that fermentation parameters in 10 L tank were defined as following： 250 r/min agitator speed, 3% inoculation amount, 2.0vvm aeration rate and seed solution of OD600 =0.8- l. 0. Under these conditions, the highest chitosanase activity in fermentation liquid was detected at 52 h during 56 h fermentation period, which reached （ 35.2 ± 0.5 ） U/mL. The maximum ehitosanase activity was detected at 60 h during a 64 h fermentation period of feed batch fermentation, which reached （46.4±0.5） U/mL. The enzyme production of feed batch process increased 32% by maintaining the mycelial morphology and extending the time of enzyme production stage. This article has made some contribution to industrial production of chitosanase.
出处
《上海海洋大学学报》
CAS
CSCD
北大核心
2015年第6期952-959,共8页
Journal of Shanghai Ocean University
基金
上海市科学技术委员会工程中心建设项目(11DZ2280300)
关键词
壳聚糖酶
发酵工艺
链霉菌
chitosanase
fermentation process
Streptomyces
