摘要
目的:观察大鼠富血小板血浆与人脱钙牙本质基质复合物修复大鼠下颌骨缺损的疗效,探讨其作为组织工程骨的有效性。方法:分别参照Urist法和Landersberg法制备脱钙牙本质基及富血小板血浆,在扫描电镜下观察两者复合物的三维空间结构。建立36只雄性SD大鼠下颌角部4 mm圆形全厚骨缺损模型,随机分为P组在骨缺损处填入富血小板血浆;D组在骨缺损处填入脱钙牙本质基质;PD组在骨缺损处填入富血小板血浆-脱钙牙本质基质复合物;空白组,不加任何材料。术后2周,6周,10周每组处死3只大鼠,摘取下颌骨,通过组织学及X线观察下颌骨组织修复情况。应用Image Pro Plus 6.0软件计算每个时间点各组标本X线片新生骨面积,计算新生骨面积占骨缺损总面积百分比。结果:术后各组标本骨缺损区均有新骨形成,成骨量随时间延长而增加。经影像学和组织学评估,各组的成骨能力依次为:PD组>D组>P组>空白组,P组和空白组之间差异无统计学意义(P>0.05),其余各组间差异有统计学意义(P<0.05),其中PD组成骨能力最强。结论:脱钙牙本质基质与富血小板血浆的复合物能促进和加速骨缺损的修复,有望成为一种新型组织工程骨。
Objective:This study aimed to observe the efficacy of repairing the rat mandibular bone defect with the compound rat platelet-rich plasma with human demineralized dental matrix and investigate preliminarily the validity of the compound platelet-rich plasma with demineralized dental matrix as the tissue engineering bone. Method: The decalcified dentin matrix and platelet rich plasma were prepared according to the methods described by Urist and Landersberg respec- tively:Three dimensional structure of the two complexes were observed by scanning electron microscope:Thirty-six male Sprague-Dawley rats were used. A 4mm full-thickness and circular defect was created through the angle of the mandible. The rats were randomly divided into P group, D group, PD group and blank group, filling in the bone defects with the platelet rich plasma,decalcified dentin matrix, and the compound rich platelet plasma with decalcified dentin matrix (blank group without any material) respectively. Three rats in each group were sacrificed in two, six and ten weeks after operation. Then the mandibles were removed and the reconstruction of mandibular tissue were evaluated by histological and X-ray observa- tion. Image Pro Plus 6.0 software was used to calculate the percentage of new bone area in each group at each time point. Result: After the operation, the bone defect area in each group had new bone formation, and the bone mass increased with time. After imaging and histological evaluation, the osteogenic ability of each group was as follows: PD group〉D group 〉 P group 〉 Blank group. There was no significant difference between the P group and the blank group (P 〉0.05), and the differ- ence between the other groups was statistically significant (P 〈0.05). The compound platelet-rich plasma with demineralized dental matrix had the strongest ability for bone formation. Conclusion: The compound platelet-rich plasma with demineral- ized dental matrix can promote and accelerate the reconstruction of bone defect, and is expected to become a new type of tis- sue engineering bone.
出处
《临床口腔医学杂志》
2015年第12期734-739,共6页
Journal of Clinical Stomatology
基金
黑龙江省博士后资助项目(LBH-Z11065)
关键词
富血小板血浆
脱钙牙本质基质
颌骨缺损
骨组织工程
platelet rich plasma
decalcified dentin matrix: mandibular defect
bone tissue engineerin