摘要
目的探讨肝癌组织中类固醇受体共激活因子-3(SRC-3)表达及其与肝癌临床病理特征的关系。方法采用免疫组织化学染色和RT-PCR检测80例肝癌组织和癌旁组织中SRC-3蛋白和mRNA表达水平。结果肝癌组织中SRC-3蛋白阳性表达率为56.25%,明显高于癌旁组织的8.75%,差异有统计学意义(P<0.05)。58例肝癌组织中SRC-3 mRNA呈高水平转录,癌旁组织仅有12例出现高水平转录,肝癌组织中SRC-3 mRNA转录水平较癌旁组织明显升高(P<0.05)。合并乙型肝炎病毒(HBV)感染肝癌患者SRC-3蛋白表达阳性率为63.49%,明显高于未合并HBV感染肝癌患者的29.41%,差异有统计学意义(P<0.05);甲胎蛋白(AFP)<400 ng·m L-1肝癌患者SRC-3蛋白表达阳性率为76.19%,明显高于AFP≥400ng·m L^(-1)肝癌患者的49.15%,差异有统计学意义(P<0.05);高分化肝癌患者SRC-3蛋白表达阳性率为88.24%,明显高于中、低分化患者的47.62%,差异有统计学意义(P<0.05)。结论 SRC-3的高表达与肝癌的疾病进展关系密切,可能成为潜在的肝癌分子标志物或治疗靶点。
Objective To investigate the expression of steroid receptor coactivator-3 （SRC-3）in the hepatocellular carcinoma tissues and its relationship with clinicopathological features. Methods immunohistochemical staining and RT-PCR were used to detect SRC-3 protein and mRNA expression levels in the 80 cases of hepatoeellular carcinoma and paraneoplastie tissues. Results The positive expression rate of SRC-3 protein in hepatocellular carcinoma tissues was 56.25% , which was significantly higher than that （ 8.75 % ） in the paraneoplastic tissues （ P 〈 0. 05 ）. SRC-3 mRNA was high level transcription in the 58 cases of hepatocellular carcinoma, and was 12 eases in the paraneoplastic tissues, SRC-3 mRNA transcription level in the hepatocellular carcinoma was significantly increased than the paraneoplastic tissue（ P 〈0.05 ）. The positive rate of SRC-3 protein expression in the patients with hepatitis B virus （HBV） infection was 63.49% , which was significantly higher than that （29.41%）of patients with non HBV infection, the difference was statistically significant（ P 〈 0.05 ） ; the positive rate of SRC-3 protein expression in the patients with alpha-fetoprotein （AFP） 〈400 ng · mL^-1 was 76.19% ,significantly higher than that（49.15% ）in the patients with AFP ≥400 ng · mL^-1, the difference was statistically significant（ P 〈 0.05 ） ; the positive rate of SRC-3 protein expression in the patients with high differentiated hepatocellular carcinoma was 88.24% , significantly higher than that （47.62%）in the patients with inmiddle and low differentiated hepatoeellular carcinoma, the difference was statistically significant （ P 〈 0.05 ）. Conclusion SRC-3 high expression is related with the canceration and progression of hepatocellular carcinoma, and may become a potential molecular marker or therapeutic target.
出处
《肿瘤基础与临床》
2015年第6期469-473,共5页
journal of basic and clinical oncology
