摘要
采用萃取浓缩-气相色谱/质谱联用技术(EE-GC/MS)对未经碱预处理与经过2%Na OH碱预处理的稻草秸秆水解液中抑制物组成成分进行了研究,旨在为解决碱预处理对木质纤维素原料生产燃料乙醇过程的抑制作用提供理论支持。研究结果表明,经过碱预处理的稻秸水解液中检测出抑制物种类共6类30种,对照组共8类23种。其中,经过碱预处理的水解液中酚类和酸类物质种类比对照组明显增多,酚类物质种类从3种增加到10种,酸类物质种类从4种增加到8种;相对含量亦比对照组显著增加,酚类物质的相对含量从10.87%增长至64.03%,酸类物质的相对含量从21.04%增加到30.43%,成为最优碱预处理条件下的水解液中的主要抑制物,其中阿魏酸和十六烷酸相对含量最高,分别为43.79%及18.13%;此外,其它类别的物质相对含量均大幅降低。综合看来,经过碱预处理的水解液中抑制物总抑制作用强于对照组。
Extraction and enrichment-gas chromatograph/mass spectrometer（EE-GC/MS） was used to investigate the inhibitor composition in rice straw hydrolysate that was pretreated by 2% Na OH and non-alkaline pretreatment sample,which could provide theoretical support for solving the inhibition problem of alkali pretreatment on producing ethanol by lignocellulose. Results showed that inhibitor species in alkali pretreatment hydrolysate were constituted of 6 classes and 30 types. However,the inhibitors in control sample were constituted of 8 classes and 23 types. The types of phenols and acids in alkali pretreatment hydrolysate were distinctly increased compared to control sample,which was increased from 3 to 10,and from 4 to 8 respectively. The relative content of phenols and acids in alkali pretreatment hydrolysate were significantly arose compared to control sample,which was grown from 10.87% to 64.03%,and from 21.04% to 30.43% respectively. Phenols and acids were two main inhibitors in alkali pretreatment hydrolysate. Ferulic acid and hexadecanoic acid exhibited the highest relative contents in alkali pretreatment hydrolysate,which were 43.79% and 18.13% respectively. In addition,the relative content of the other classes of inhibitors were obviously decreased. The total inhibition effect in alkali pretreatment hydrolysate was much heavier than that in control sample.
出处
《环境科学与技术》
CAS
CSCD
北大核心
2015年第10期21-26,共6页
Environmental Science & Technology
基金
国家自然科学基金(31400513)
国家高等学校博士点基金(20120076120019)
上海市自然科学基金面上项目(12ZR1409100)
关键词
气质联用法
萃取浓缩
抑制作用
预处理
稻草秸秆
水解液
gas chromatography-mass spectrometry（GC/MS）
extraction-enrichment
inhibition
pretreatment
rice straw
hydrolysate
