摘要
在云南印楝资源全面调查的基础上,以实生株系的种子为研究对象,运用HPLC技术分析种子印楝素组分含量,采用描述统计、方差分析、系统聚类和重复力估计,定性、定量分析印楝实生株系种子品质的遗传多样性及稳定性。结果表明:云南引种栽培印楝实生株系的种子品质存在广泛变异,种子印楝素A、B、AB含量和印楝素A与印楝素B含量比的变异幅度分别为0.28%~0.85%、0.04%~0.39%、0.37%~1.15%和1.69~8.25;种子印楝素A、B、AB含量分为3个类型:高含量型(az A≥0.69%、az B≥0.25%、az AB≥0.92%)、低含量型(az A≤0.44%、az B≤0.10%、az AB≤0.51%)和中等含量型(az A=0.68%~0.45%、az B=0.24%~0.11%、az AB=0.91%~0.52%);按此标准将高印楝素AB含量型分为"印楝素AB优异型(az AB≥1.15%)"、"印楝素A优、B劣型"(az A≥0.85%、az B≤0.21%)、"印楝素A劣、B优型"(az A≤0.67%、az B≥0.39%)和"印楝素AB含量高型"(az AB=1.05%~0.92%);反映在重复力上,印楝实生株系种子印楝素A、AB的重复力分别为0.206 3、0.325 2,都属于"中"重复力性状。"印楝素AB优异型"即具有优异种子品质性状的植株是印楝药用原料林培育的繁殖材料;"印楝素A优、B劣型"和"印楝素A劣、B优型"即种子品质性状优缺点互补的植株是药用印楝品质育种的杂交亲本。
Based on fully investigation of neem resources in Yunnan, the azadirachtin content of seedling colonies were tested by HPLC. The genetic diversity and stability of Azadirachta indica seed quality characters were qualitatively and quantitatively analyzed by means of descriptive statistics, ANOVA, hierarchical clustering, and repeatability estimation. The results showed that ： （1） The seed quality varied in a wide range, with variation of azadirachtin A, azadirachtin B, azadirachtin A＆B, and ratio of azadirachtin A to B as 0.28%-0.85%, 0. 04% - 0. 39% , 0.37% - 1.15% and 1.69 - 8.25, respectively. （2） The azadirachtin content of seeds could be divided into three types ： High （ azA ≥0.69% , azB ≥0.25 % , and azAB ≥0.92% ） , Low （ azA ≤0.44% , azB ≤0.10% , and azAB ≤0.51%）, and Medium （azA = 0. 68% - 0. 45% , azB=0.24%-0. 11%, and azAB=0.91%-0.52%）. Tile type ＂High＂ could be further divided into ＂Superior azA＆B （ azAB ≥ 1.15 % ） ＂, ＂ Superior azA with poor azB （ azA≥ 0.85 % , azB ≤ 0.21% ） ＂ , ＂Superior azB with poor azA （ azA≤0.67% , azB≥ 0.39% ） ＂, and ＂ HighazA＆B （ azAB = 1.05% ^-~0.92% ）＂ （3） The repeatability of azA and azAB were 0.206 3 and 0.325 2 respectively, with a ＂medium＂ repeatability. （4） The seeds of type ＂Superior azA＆B＂ were the ideal materials for officinal neem breeding, and the type ＂Superior azA with poor azB＂ and ＂Superior azB with poor azA＂ could be cross parents in further officinal neem breeding to provide potential complementary.
出处
《林业科学研究》
CSCD
北大核心
2015年第6期767-774,共8页
Forest Research
基金
国家"十一.五"科技支撑计划项目(2006BAD18B0302)
国家"十二.五"科技支撑计划项目(2012BAD21B04)
国家自然科学基金(31270710)
关键词
印楝
遗传资源
印楝素
遗传多样性
重复力
Azadirachta indica
genetic resources
azadirachtin
genetic diversity
repeatability
