摘要
目的：建立化痔胶囊中柚皮苷、新橙皮苷的高效液相色谱法（HPLC）含量测定方法。方法：以乙腈-0.5%磷酸（42∶58）为流动相,检测波长为283 nm,采用HPLC对化痔胶囊中柚皮苷、新橙皮苷的进行含量测定。结果：柚皮苷和新橙皮苷分别在0.4658～9.3160μg（r=0.9997）、0.2834～5.6680μg（r=0.9994）,进样量与峰面积呈良好的线性关系,平均加样回收率分别为99.89%、99.07%,RSD（n=6）分别为1.32%、1.85%。结论：方法简便、准确、灵敏、重复性好,可作为化痔胶囊中的柚皮苷和新橙皮苷含量控制方法。
Objective： It is to establish HPLC determination of naringin in hemorrhoids capsule in the new method of the content of hesperidin. Methods： Using high performance liquid chromatography （HPLC） method for hem- orrhoids capsule of naringin and new content determination of hesperidin, with a 0.5% acetonitfile phosphate （42 ： 58） as mobile phase, detection wavelength ： 283 nm. Results： New naringin and hesperidin in 0. 4658 ～ 9.3160 mug （ r = 0. 9997 ）, 0.2834 ～ 5. 6680 mug （ r = 0.9994） sample had good linear relationship with the peak area, the average sample recovery rate was 99.89%, 99.07%, and RSD （n =6） were 1.32%, 1.85% respectively. Conclusion： The method is simple, accurate, sensitive and reproducible, and can be used as the main new capsule of naringin and hesperidin content control method.
出处
《安徽科技学院学报》
2015年第5期20-23,共4页
Journal of Anhui Science and Technology University
基金
安徽省高等学校教学质量与教学改革工程实训中心建设项目(20101460)
关键词
化痔胶囊
柚皮苷
新橙皮苷
Hemorrhoids capsule
Naringin
New hesperidin
