摘要
以某污水处理厂初沉污泥和生物污泥为研究对象,研究了Fe Cl_3及Al Cl_3不同投加浓度对污泥中温厌氧消化系统硫化氢产生的抑制作用,利用16S r DNA高通量测序技术及定量PCR技术分析了消化污泥中硫酸盐还原菌的变化。结果表明,Fe Cl_3及Al Cl_3的投加对污泥厌氧消化系统中H_2S的产生都具有抑制作用,Fe Cl_3的抑制效果优于Al Cl_3。随Fe Cl_3及Al Cl_3投加浓度的增加沼气中H_2S浓度下降,Fe Cl_3投加浓度为150 mg/L时,H_2S含量降至零;通过分析硫酸盐还原菌的序列数并采用定量PCR测定样品中的dsr B基因拷贝数,投加Fe Cl_3及Al Cl_3的消化系统的硫酸盐还原菌数量在实验第9天大幅降低,这与该系统硫化氢浓度变化规律一致,可以推断,Fe Cl_3及Al Cl_3对硫酸盐还原菌的抑制是沼气中硫化氢含量降低的重要原因之一。
Using the primary sedimentation sludge and biological sludge from one sewage treatment plant,this study investigated the inhibition effect of a range of FeCl3 and AlCl3 doses on H2S from sludge mesotherm anaerobic digestion systems. The change in sulfate-reducing bacteria（ SRB） in the digestion system was also determined by 16 S rDNA high-throughput sequencing and quantitative PCR. The experimental results indicated that the dosed FeCl3 and AlCl3 significantly inhibited the generation of H2S in biogas. The concentration of H_2 S in biogas decreased when the FeCl3 and AlCl3 dose increased and it decreased to zero when the FeCl3 dose was 150 mg/L. The analysis of the sequence quantities and dsr B gene copies indicated that the SRB quantity rapidly decreased on the 9th day of the experiment in all the digestors dosed FeCl3 or AlCl3,which was consistent with the H_2 S variations in the corespongding digestion sytstem. The study concluded that the inhibition of SRB by FeCl3 and AlCl3 explains the reduction of H2S in a sludge anaerobic digestion system as a function of FeCl3 and AlCl3 dose.
出处
《环境工程学报》
CAS
CSCD
北大核心
2015年第12期5907-5914,共8页
Chinese Journal of Environmental Engineering
