摘要
目的改进检测食品中苏丹红染料的高效液相色谱法的前处理方法,使方法更加简便快速。方法以正己烷为提取溶剂,提取液经中性氧化铝层析柱净化,经高效液相色谱C18柱进行分离,外标法定量。结果苏丹红Ⅰ~Ⅳ在0.5~10.0μg/ml的线性范围内线性关系良好,苏丹红Ⅰ~Ⅳ的检出限分别为0.012 mg/kg、0.022 mg/kg、0.013 mg/kg和0.019 mg/kg,加标回收率75.9%~92.0%,相对标准偏差1.99%~4.31%。结论改进后的前处理方法比原方法节省试剂,降低了环境污染,且方法快速、准确,符合痕量分析要求,可用于食品中苏丹红染料的残留检测。
Objective To improve pretreatment method of detection for Sudan Ⅰ-Ⅳ in food by high performance liquid chromatography. Methods Hexane was applied as extraction solvent, purified by neutral alumina column, separated by HPLC column, and quantified by external standard method. Results SudanⅠ-Ⅳ had a good linear relationship within the linear range from 0.5 to 10.0 μg/ml, with the detective limit of 0.012 mg/kg, 0.022 mg/kg, 0.013 mg/kg, and 0.019 mg/kg, respectively, the recoveries from 75.9% to 92.0%, and relative standard deviation from 1.99% to 4.31%. Conclusions The improved method can save reagent, reduce pollution in environment. It is simple, fast and accurate, can be used in detection for SudanⅠ-Ⅳ in foods.
出处
《疾病预防控制通报》
2015年第6期57-58,86,共3页
Bulletin of Disease Control & Prevention(China)
关键词
高效液相色谱
食品
苏丹红染料
High performance liquid chromatography
Food
SudanⅠ-Ⅳ
