摘要
空气中的病原微生物容易随气流快速扩散而造成大范围疾病疫情,现有技术方法均难以对其进行快速监测分析。该文以微流控技术为基础,结合空气流体芯片结构设计和液体LAMP扩增方法,构建了一种用于捕获和检测空气金黄葡萄球菌的微流控芯片系统,能在1.5 h内完成分析,结果肉眼可见,具有速度快、价格低和便携的优势,特别适合于对空气气流的现场快速检测。
Airborne pathogen spread fast by force of air flow, which causes respiratory infectious diseases. While,conventional techniques can hardly provide effective ways for rapid detection of the airborne pathogens. Herein, a microfluidic chip-based technique with chip design for air aerodynamics combined with liquid phase LAMP amplifications are reported. The presented chip is capable of capture and detection of airborne Staphylococcus aureus in 1.5 h. The result can be observed by naked eyes. The microfluidic chip was portable, fast and low-price, indicating its potentially application in point-of-care detections of airborne pathogen.
出处
《水动力学研究与进展（A辑）》
CSCD
北大核心
2015年第6期619-623,共5页
Chinese Journal of Hydrodynamics
基金
国家自然科学基金项目(81501833)
2015国家卫生计生委卫生和计划生育卫生监督中心微流控芯片快检委托研究项目
中央高校基本科研业务费专项资金资助(1306-852010)~~
