摘要
为探讨猪源性成分检测可行的方法,利用SYBR Green Ⅰ Real time PCR对动物源性食品中猪源性成分进行测定。根据猪mtDNA保守序列设计特异性引物,对牛、羊、猪的动物源性食品进行扩增;克隆目的基因片段,并以此为标准品,优化反应条件和反应体系,提高灵敏度;反复检测不同样品,测试稳定性。结果表明SYBR GreenⅠReal time PCR技术能够特异性的检测到动物源性食品中猪源性成分,引物特异性强、稳定性可靠,灵敏度达到1×10^(-6)ng/μL,建立了动物源性食品中猪源性成分的快速、精准、经济、便捷的检测方法。
To establish a specific method detecting the swine derived ingredients in some animal derived foods,a real-time PCR was established according to the conserved sequences of swine mtDNA, specific primers weredesigned and the animal derived products of bovine, ovine and swine were amplified. The target genes werecloned and reaction system was optimized to improve detection sensitivity. Different samples were testedrepeatedly to test the stability of the detection method. The results demonstrated that only swine derivedingredients in those animal derived foods were detected. And the detection sensitivity reached 1×10-6ng/μL.The established method was excellent for identification of swine derivatives in animal derived foods as it wasaccurate, cheap, convenient and fast.
出处
《中国农学通报》
2015年第36期259-264,共6页
Chinese Agricultural Science Bulletin
基金
2014西北民族大学本科生科研创新项目"SYBR Green Ⅰ Real time PCR对牛
羊
鸡
马
鸭
猪源性成分定性
定量分析研究"(URIP14149)
