摘要
建立了一种同时测定洗发水中水杨酸、吡硫翁锌、酮康唑、氯咪巴唑和吡罗克酮乙醇胺盐5种去屑剂的高效液相色谱法。采用C18柱,以乙腈-甲醇-0.01 mol/L的磷酸二氢钾溶液（含乙二胺四乙酸二钠0.5 mmol/L,磷酸调节p H=4）梯度洗脱分离各个组分,进样体积5μL,柱温35℃,检测波长230 nm。结果表明：水杨酸、吡硫翁锌、酮康唑和氯咪巴唑的线性范围为10~200 mg/L,吡罗克酮乙醇胺盐的线性范围为20~400 mg/L,方法的线性关系良好。水杨酸、吡硫翁锌、酮康唑、氯咪巴唑和吡罗克酮乙醇胺盐的检出限分别为0.26,0.53,0.26,0.26和2.0 mg/L,平均回收率为96.04%~98.42%。采用该方法对83件市售去屑产品进行检测：有57件检出吡硫翁锌,18件检出吡罗克酮乙醇胺盐,7件检出水杨酸,3件检出氯咪巴唑,未检出禁用物质酮康唑。所有产品去屑剂含量均符合标准要求。
A high performance liquid chromatography method with gradient elution was established for simultaneous determination of anti- dandruff agent salicylic acid,pyrithione zinc,ketoconazole,climbazole and octopirox in shampoo formulations. A C18 column was used as stationary phase,and mixture containing acetonitrile- methanol aqueous solution（ 0. 01 mol / L KH2PO4 and 0. 5 mmol / L EDTA- 2Na,p H adjusted to4 with H3PO4） eluted by gradient was used as mobile phase in the HPLC at the wavelength of 230 nm.Operating temperature was 35 ℃. Injection volume was 5 μL. The linearity range of salicylic acid,pyrithione zinc,ketoconazole and climbazole is 10 ~ 200 mg / L. The linearity range of octopirox is 20 ~ 400 mg / L. The detection limits for salicylic acid,pyrithione zinc,ketoconazole,climbazole and octopirox are 0. 26,0. 53,0. 26,0. 26 and 2. 0 mg / L respectively. The spiked recovery of matrix for salicylic acid,pyrithione zinc,ketoconazole,climbazole and octopirox varies from 96. 04% to 98. 42%. Among test results of 83 shampoo samples from market,pyrithione zinc appears in 57 samples; octopirox appears in 18 samples; salicylic acid appears in 7 samples; climbazole appears in 3 samples; banned substance ketoconazole is not detected. The contents of anti- dandruff agents in all samples meet the relevant standard requirements.
出处
《日用化学工业》
CAS
CSCD
北大核心
2015年第12期715-718,共4页
China Surfactant Detergent & Cosmetics
关键词
洗发水
去屑剂
高效液相色谱法
shampoo
anti-dandruff agent
high performance liquid chromatography
