摘要
[目的]观察金丝桃苷对小鼠抗氧化能力和免疫功能的影响。[方法]将BALB/c小鼠随机分成5组:空白对照组、免疫抑制组、给药组(包括低剂量给药组和高剂量给药组)和阳性对照组,测定胸腺指数和脾脏指数,机体过氧化物MDA、H_2O_2水平,总抗氧化能力T-AOC及其酶T-SOD,以及MPO、XOD、GSH-PX和POD等与抗氧化能力相关酶的活性。[结果]与空白对照组相比,免疫抑制组的胸腺指数、脾脏指数、T-AOC、T-SOD、MPO、GSH-PX及POD活性显著降低,MDA、H_2O_2和XOD活性显著升高。给予金丝桃苷治疗后,与免疫抑制组相比,胸腺指数、脾脏指数、T-AOC、T-SOD、MPO、GSH-PX及POD的活性显著升高,MDA、H_2O_2和XOD活性显著降低。[结论]金丝桃苷能通过提高免疫抑制小鼠体内胸腺指数、脾脏指数,降低机体过氧化物MDA、H_2O_2,提高T-AOC、T-SOD、GSH-PX及POD活性来清除机体过氧化物,降低XOD活性来减少过氧化物的产生,以此提高小鼠抗氧化能力,增加机体免疫功能。
[Objective] To investigate effects of hyperoside on the antioxidant capability and immune function of mice.[Method]The BALB / c mice were randomly divided into 5 groups： blank control group,immunosuppressed group,drug group,positive control group. Then the level of MDA,H_2O_2,thymus index,spleen index T-AOC,T-SOD,MPO,XOD,GSH-PX and POD were measured.[Result]The expression of thymus index,spleen index,T-AOC,T-SOD,MPO,GSH-PX and POD decreased significantly,and the level of MDA,H_2O_2 and XOD increased significantly compared with blank control group. After treatment with hyperoside,the level of thymus index,spleen index,T-AOC,T-SOD,MPO,GSH-PX and POD increased significantly,while the level of MDA,H_2O_2 and XOD reduced significantly compared with the immunosuppressed group.[Conclusion] Hyperoside could improve the antioxidant capability and immune function by increasing the level of thymus index,spleen index,T-AOC,TSOD,MPO,GSH-PX and POD and decreasing the level of MDA,H_2O_2,and XOD in immunosuppressed mice.
出处
《安徽农业科学》
CAS
2015年第34期170-172,共3页
Journal of Anhui Agricultural Sciences
关键词
金丝桃苷
免疫抑制
抗氧化能力
免疫功能
Hyperoside
Immunosuppression
Antioxidant capability
Immune function
