摘要
建立UPLC-QTRAP-MS/MS同时测定玄参中10种核苷类成分含量的方法,分析不同采收期玄参中核苷类成分动态积累变化。采用UPLC-QTRAP-MS/MS技术同时测定玄参样品中10种核苷类成分的含量。玄参核苷类成分中,以鸟苷、尿苷、腺苷、尿嘧啶含量较高;不同采收期玄参核苷类成分含量有所差异,11月份核苷含量相对较高。为探究玄参药材的品质形成机制及确定药材适宜采收期提供基础资料。
The goal of this research was to establish an UPLC-QTRAP-MS/MS method for the determination of 10 kinds of nucleosides and nucleobases in Screphulariae Radix and to investigate the dynamic accumulation of nucleosides and nucleobases in different harvesting periods of Scrophulariae Radix. UPLC-QTRAP-MS/MS was applied for the analysis of 10 kinds of nucleosides and nucleobases in Scrophulariae Radix. Compared with other nucleosides and nucleobases in Scrophulariae Radix, the contents of guanosine, uridine, adenosine and uracil were relatively higher. There were differences in the contents of nucleosides and nucleobases in Scrophulariae Radix at different harvesting periods ;the contents were relatively higher in those grown in November. This study provided a scientific basis for the investigation of the influence of the quality formation mechanism of Scrophulariae Radix as weU as the determination of the appropriate harvesting time,
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2015年第12期2079-2083,共5页
Natural Product Research and Development
基金
江苏省高校优势学科建设工程资助项目(ysxk-2014)
