摘要
A method for in vitro culture and rapid propagation of Chirita ophio- pogoides was developed using leaves as explants in this study, The results indicat- ed that the medium MS＋6-BA 0.1 mg/L＋NAA 0.1 mg/L was suitable for bud induc- tion and seedling regeneration from leaves in primary culture. The media MS＋0.5 mg/L 6-BA＋0,1 mg/L NAA＋10% banana＋5% potato and MS＋0.5 mg/L 6-BA＋0.5 mg/L NAA＋2% banana were very suitable for callus multiplication and seedling hardening in subculture, and the proliferation coefficients were 7,9 and 5.6 per 60 d respec- tively. The optimal rooting medium was MS and the rooting rate was 100% on day 30 of culture. The rooted plantlets of C. ophiopogoides were transplanted in green- house with humus soil and 92.5% survived. Theoretically, using the rapid propaga- tion system, about 20 176 seedlings can be reproduced from a sterile plantlet in a year.
以叶片为外植体,对条叶唇柱苣苔(Chirita ophiopogoides)进行离体培养与快速繁殖研究。结果表明,培养基MS+0.1 mg/L 6-BA+0.1 mg/L NAA适用于初代培养中的芽诱导和植株再生;培养基MS+0.5 mg/L 6-BA+0.1mg/L NAA+10%香蕉+5%马铃薯和MS+0.5mg/L 6-BA+0.5 mg/L NAA+2%香蕉分别适用于继代增殖和壮苗培养,繁殖系数分别为7.9倍/60 d和5.6倍/60 d;培养基MS适用于生根培养,培养30 d后生根率达到100%;以腐质土为基质,将条叶唇柱苣苔生根试管苗移栽到大棚,成活率达92.5%。根据上述快繁技术,理论上每株试管苗每年可繁殖条叶唇柱苣苔种苗20 176株。
基金
Supported by National Natural Science Foundation of China(31160055)
Key Science and Technology Research and Development Program of Guangxi(Gui Ke Gong0992003B-31)~~
