摘要
目的:建立旱田草药材的定性定量方法,考察广西不同产地、不同采收时节的药材质量。方法:采用TLC法对旱田草进行定性鉴别,展开剂为乙酸丁酯-甲醇-甲酸-水(6∶1.5∶1.5∶1);采用HPLC法对旱田草中的毛蕊花糖苷进行含量测定,使用Waters Sun Fire C18色谱柱(4.6 mm!250 mm,5μm),柱温35℃,以甲醇-0.4%磷酸溶液(32∶68)为流动相,流速1.0 m L·min^(-1),检测波长334 nm;参照中国药典2010年版一部测定水分、灰分和浸出物。结果:薄层色谱中斑点清晰,分离度好;毛蕊花糖苷质量浓度在2.002~40.04μg·m L^(-1)(r=1.000 0)范围内线性关系良好,加样回收率为98.61%(RSD=1.7%,n=6);所测的12批样品含量在0.196~16.76 mg·g^(-1)之间。结论:本文建立的方法可用于瑶药旱田草的质量控制。
Objective： To establish a qualitative and quantitative method for Lindernia ruellioides（ Colsm.） Pennell,and investigate the quality of this medicine from different habitats and harvest seasons in Guangxi.Methods： The TLC method was used for qualitative identification and butyl acetate-methanol-formic acid-water（ 6∶ 1. 5∶ 1. 5∶ 1） was used as the developing agent; and the determination of verbascoside was conducted by HPLC using a column of Waters Sun Fire C18（ 4. 6 mm × 250 mm,5 μm）,the mobile phase was methanol-0. 4% phosphoric acid（ 32∶ 68） at a flow rate of 1. 0 m L·min-1; the detection wavelength was 334 nm,and the column temperature was 35 ℃. The contents of moisture,total ash and ethanol-soluble extractives were determined with the methods in Chinese Pharmacopeia（ Ch P 2010）. Results： The spot of verbascoside could be well identified by TLC. In the system of HPLC,verbascoside showed good linear relationship in the ranges of 2. 002-40. 04 μg·m L-1（ r = 1. 000 0）,The average recovery was 98. 61%（ RSD = 1. 7%,n = 6）. The contents in 12 batches of samples were between0. 196 mg·g-1to 16. 76 mg·g-1. Conclusion： The qualitative and quantitative method established can be used for the quality control of Lindernia ruellioides（ Colsm.） Pennell.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2015年第12期2143-2147,共5页
Chinese Journal of Pharmaceutical Analysis
关键词
瑶药
旱田草
毛蕊花糖苷
薄层色谱
高效液相色谱
Yao medicine
Lindernia ruellioides（Colsm.） Pennell
verbascoside
TLC
HPLC
