摘要
目的:探讨松萝酸对小鼠H22肿瘤生长的影响及其作用机制。方法:昆明种小鼠前腋下皮下接种H22细胞,第2天腹腔注射松萝酸,每3 d测量1次肿瘤体积。用免疫组化法检测微血管密度(MVD);ELISA法检测小鼠血清和人脐静脉血管内皮细胞(HUVEC)细胞培养基上清中血管内皮生长因子(VEGF)和碱性成纤维细胞生长因子(b FGF)水平;MTT法检测松萝酸对人脐静脉血管内皮细胞(HUVEC)体外增殖的影响。结果:松萝酸组小鼠H22肿瘤体积、重量增长明显较模型组缓慢(P<0.05),同时松萝酸组的肿瘤微血管密度显著减少(P<0.05),松萝酸对HUVEC体外增殖有显著抑制作用。松萝酸可显著降低HUVEC细胞和小鼠血清VEGF和b FGF水平(P<0.01)。结论:松萝酸可显著抑制小鼠H22肿瘤生长及血管生成,且能抑制VEGF和b FGF表达和HUVEC体外增殖。松萝酸对VEGF和b FGF分泌的抑制作用可能是其抗H22肿瘤及抗血管生成的一个重要机制。
Objective： To investigate the growth inhibitory effect of usnic acid on H22 tumor cells in mice and the underlying mechanism. Methods： The H22 cells were armpit hypodermically inoculated in KM mice, usnic acid started to be injected intraperitoneally on the next day, and then the tumor volume was measured every 3 days. The microvessel density （MVD） was detected by immunohistochemistry. The levels of VEGF and bFGF were detected by ELISA. The proliferation of HUVEC cells was detected by MTV assay. Results： The volume and weight of H22 tumor were increased more slowly in the usnic acid group than those in the model group （P 〈 0.05）. The microvessel density of the usnic acid group was significantly reduced （P 〈 0.05） and usnic acid had significant inhibitory effect on the proliferation of HUVEC. Usnic acid could reduce the serum levels of VEGF and bFGF of HUVEC cells （ P 〈 0.01 ）. Conclusion： Usnic acid can significantly inhibit the growth and angiogenesis of H22 tumor cells, the expression of VEGF and bFGF, and the proliferation of HUVEC. The mechanism of the growth and angiogenesis inhibitory effect on H22 tumor cells may be related to the expression inhibitory effect of usnic acid on VEGF and bFGF.
出处
《中国药师》
CAS
2016年第1期29-32,共4页
China Pharmacist
基金
甘肃省自然科学基金(编号:148RJZA058)
