摘要
用PCR的方法扩增得到斜纹夜蛾核多角体病毒 (SpltMNPV)ORF137全长基因 (Splt137)。将其克隆到原核表达载体pQE30上 ,并转化大肠杆菌M15 ,在IPTG诱导下表达了与预期相对分子质量相符的一个 2 7× 10 7的蛋白质 ,经过聚丙烯酰氨凝胶电泳纯化 ,免疫家兔制备了抗Splt137抗体。Western免疫印迹分析表明 ,该抗体适合用作Splt137蛋白的进一步分析。
One of Spodoptera litura multinucleocapsid nuclear polyhdrosis virus ( Splt MNPV) unique ORFs ORF137 ( Splt137 ) was obtained by PCR method from genome DNA. The PCR products were cloned into the pGEM_T easy vector to get the recombinant plasmid (pGT_SL137). The Splt 137 was recombined in vitro with expression vector pQE30 and transformed into E coli M15. The M15 strain, containing Splt137 recombinant plasmid, expressed a 27 000 protein in agreement with the prospected molecule after the induction with IPTG. The expressed protein was used to raise antiserum. Western blot analysis indicated that the antiserum could react with the corresponding protein, and was suitable to be used for further analysis of Splt137 protein.
出处
《中山大学学报（自然科学版）》
CAS
CSCD
北大核心
2002年第4期75-77,共3页
Acta Scientiarum Naturalium Universitatis Sunyatseni
基金
国家"973"专题资助项目 (G2 0 0 0 0 16 2 2 0 9)
