摘要
目的 探求用软骨膜复合移植的方法形成自体软骨的可行性。 方法 将兔自体耳软骨膜包裹异体耳软骨脱细胞基质材料复合游离移植于兔背部皮下 (Ⅰ组 ) ,并在移植物附近皮下游离移植双层耳软骨膜 (Ⅱa组 )及单纯脱细胞的异体耳软骨基质 (Ⅱb组 )作为对照。在术后 3周、6周分别取标本进行研究。 结果 在 3周、6周时 ,Ⅰ组与Ⅱa组成软骨细胞的增殖变化情况差异有显著性 (P <0 .0 5 ) ,Ⅰ组比Ⅱa组成软骨细胞的增殖明显旺盛 ,有的已经向软骨细胞转化 ,并有少量细胞外基质分泌 ,基质内软骨囊出现。Ⅱb组无成软骨细胞及软骨囊出现。 结论 自体耳软骨膜与异体耳软骨脱细胞后的基质材料复合移植促进自体软骨生成。提示该基质材料有促进软骨膜细胞分化和生长的作用。
Objective To study the possibility of generating cartilage with a free composite graft of perichondrium and matrix. Methods A composite graft of a free perichondrium flap wrapped around acellular auricular cartilage matrix was implanted in the back subcutaneously of young New Zealand rabbits (GroupⅠ). As controls,double folded perichondrium free flap (GroupⅡa) and acellular auricular cartilage matrix without perichondrium cover (GroupⅡb) were implanted. Results 3 weeks and 6 weeks after implantation , obvious differences of chondroblasts derived from the perichondrium were observed between the GroupⅠand GroupⅡa (P<0.05), and the chondroblasts in GroupⅠwere more productive than that in GroupⅡa ,some cells have transformed into chondrocytes with secretion of matrix , even the appearance of cartilage cysts in GroupⅠ. No chondroblasts and cartilage appeared in GroupⅡb. Conclusions A composite graft consisting of acellular auricular cartilage matrix in an envelop of perichondrium is capable of heterotopic cartilage formation,and that acellular auricullar cartilage matrix serves as inductor for the perichondrium to produce chondrogenetic cells and promotes cartilage formation were proposed.
出处
《中国微创外科杂志》
CSCD
2002年第4期244-246,共3页
Chinese Journal of Minimally Invasive Surgery
