摘要
背景:Toll样受体及其信号通路在自身免疫性疾病、超敏反应、炎症反应、细胞凋亡及移植排斥反应中发挥重要的作用,其在过敏性紫癜患儿免疫发病机制中的地位尚未完全阐明。目的:探讨过敏性紫癜患儿外周血单核细胞TLR2的表达及其与免疫应答的相关性。方法:64例过敏性紫癜患儿分为2组,即过敏性紫癜无肾损害组(36例)、过敏性紫癜肾损害组(28例),选择同期入院的30例健康体检儿童设置为对照组,采用流式细胞术检测外周血单核细胞TLR2表达,荧光定量PCR技术检测外周血单核细胞TLR2 mR NA相对表达量,ELISA检测血浆干扰素γ、白细胞介素4水平,ELISA法测定Treg细胞分泌的转化生长因子β、白细胞介素10水平。结果与结论:过敏性紫癜组干扰素γ、干扰素γ/白细胞介素4水平均显著低于对照组(P<0.05),白细胞介素4水平显著高于对照组(P<0.05);过敏性紫癜组TLR2 mR NA和蛋白表达显著高于对照组(P<0.05);过敏性紫癜肾损害组TLR2 mR NA和蛋白表达显著高于过敏性紫癜无肾损害组(P<0.05);过敏性紫癜组患者白细胞介素10和转化生长因子β表达显著高于对照组(P<0.05)。结果表明过敏性紫癜患儿外周单核细胞TLR2mR NA及蛋白表达增高,且患儿存在免疫表达失衡现象,TLR2参与过敏性紫癜免疫发病机制,转化生长因子β表达量能够评估Treg的免疫应答,可作为过敏性紫癜患儿诊断、治疗及预后参考依据。
BACKGROUND： Toll-like receptor（TLR） and its signaling pathway play an important role in autoimmune diseases, hypersensitivity, inflammation, apoptosis and transplant rejection; however, its effects on immune pathogenesis of Henoeh-Schonlein purpura in children have not been fully elucidated.OBJECTIVE： To investigate the TLR2 expression in peripheral blood mononuclear cells in children with Henoeh-Schonlein purpura and its correlation with immune response. METHODS： Sixty-four children with Henoeh-Schonlein purpura were divided into two groups： non-renal damage group（n=36） and renal damage group（n=28）. Meanwhile, another 30 healthy children subjected to health examination acted as control group. Flow cytometry and florescent quantitative PCR were employed to detect TLR2 protein and m RNA expression in peripheral blood mononuclear cells, respectively. ELISA was used to detect plasma interferon-γ and interleukin-4 levels and transforming growth factor β and interleukin-10 levels secreted from Treg cells. RESULTS AND CONCLUSION： Levels of interferon-γ and interferon-γ/interleukin-4 in the children with Henoeh-Schonlein purpura were significantly lower than those in the control group（P〈0.05）, while the level of interleukin-4 was higher than the control group（P〈0.05）. The expression of TLR2 protein and m RNA was significantly higher in the Henoeh-Schonlein purpura children than the healthy children（P〈0.05） and significantlyhigher in the renal damage group than the non-renal damage group（P〈0.05）. Compared with the control group, the levels of interleukin-10 and transforming growth factor β were significantly higher in the children with Henoeh- Schonlein purpura（P〈0.05）. These findings indicate that Henoeh-Schonlein purpura children have increased levels of TLR2 protein and m RNA in the peripheral blood mononuclear cells, and exhibit immune imbalance. TLR2 is involved in the pathogenesis of Henoeh-Schonlein purpura, and transforming growth factor β can be used to evaluate Treg immune response and provide reference for diagnosis, treatment of prognosis of Henoeh-Schonlein purpura children.
出处
《中国组织工程研究》
CAS
北大核心
2015年第45期7356-7361,共6页
Chinese Journal of Tissue Engineering Research
