摘要
背景:如果一种提取液与细胞共培养后,能增加细胞周期的S期,减少细胞凋亡百分比,则证明这种提取液有促细胞增殖的作用。目的:验证鸡卵清提取液不同组分对293T细胞的增殖及细胞生长周期及细胞凋亡的影响。方法:用超滤管将鸡卵清提取液分离为大于10 ku和小于10 ku和大于3 ku和小于3 ku的成分,用于与细胞共培养,培养3 d后,检测细胞增殖,细胞周期与细胞凋亡情况。结果与结论:鸡卵清提取液中小于10 ku和小于3 ku的成分有促细胞增殖的作用,同时有促进细胞周期S期百分比增加,细胞凋亡百分比减少的作用。结果提示鸡卵清提取液中小于10 ku和小于3 ku的成分有促细胞增殖的作用,还有促进细胞周期S期百分比增加,细胞凋亡百分比减小的作用。
BACKGROUND：If an extract can prolong the S phase and reduce the percentage of apoptosis after co-culture with cels, it is proved that the extract is able to promote cel proliferation. OBJECTIVE： To prove the effects of chicken egg-white extracts with different components on the proliferation, cel cycle and apoptosis of 293T cels. METHODS： An ultrafiltration unit was used to separate chicken egg-white extracts into different components that were 〉 10 ku, 3 ku and 〈 3 ku to co-culture with cels for 3 days. Then, cel proliferation, cel cycle and cel apoptosis were detected. RESULTS AND CONCLUSION：Chicken egg-white extract components of 〈 10 ku and 〈 3 ku could promote cel proliferation, increase the percentage of S-phase cels and reduce the percentage of apoptosis. In conclusion, chicken egg-white extract components that are 〈 10 ku and 〈 3 ku can promote cel proliferation, as wel as increase the percentage of S-phase cels and reduce apoptosis percentage.
出处
《中国组织工程研究》
CAS
北大核心
2015年第46期7451-7455,共5页
Chinese Journal of Tissue Engineering Research
基金
国家支撑计划(2014BAI01B01)
云南省科技计划项目(2013CA005)资助的课题~~
