摘要
背景:纳米银具有显著的抗菌性能,纳米羟基磷灰石具有良好的生物活性及机械强度,将两者混合既可促进骨形成又具有抗菌性能。目的:观察纳米银与纳米羟基磷灰石混合填塞在即刻种植骨结合过程中的作用。方法:拔除9只新西兰大白兔上下颌4个切牙后,随机分为实验组(n=6)与对照组(n=3),实验组在拔牙窝内填塞纳米银与纳米羟基磷灰石混合物,对照组在拔牙窝内填塞纳米羟基磷灰石,两组均即刻植入钛螺纹钉。植入后4,8,12周取完整上下颌骨标本,进行大体观察、扭矩实验测试、X射线骨密度分析及组织学观察。结果与结论:实验组不同时间点的新生骨灰度值、最大扭矩值均高于对照组(P<0.05)。植入12周内,实验组成骨速度及新生骨组织成熟度均高于对照组,且始终无炎细胞浸润;对照组植入4周时可见大量炎细胞浸润,植入8周时仍可见少量炎细胞。表明纳米银与纳米羟基磷灰石混合植骨有更好的抗菌作用、生物相容性和骨诱导能力,能够加快骨结合,促进成骨,较单纯使用纳米羟基磷灰石效果好。
BACKGROUND：Nanosilver has significant antibacterial properties, and nanohydroxyapatite has good biological activity and mechanical strength, while their mixture cannot only promote bone formation but also have antibacterial properties. OBJECTIVE：To observe the effect of nanosilver and nanohydroxyapatite mixed filing on the osseointegration of immediate implants. METHODS： Nine New Zealand white rabbits were randomly divided into experimental group （n=6） and control group （n=3） after four incisors from the upper and lower jaw were extracted. The mixture of nanosilver and nanohydroxyapatite was filed into the tooth socket in the experimental group, while nanohydroxyapatite was filed into the tooth socket in the control group. Titanium screw was immediately implanted into both groups. The intact＆nbsp;maxila and mandibular specimens were harvested at the 4th, 8th, and 12th weeks after operation. Gross observation, X-ray bone density analysis, torque test and histological observation were conducted. RESULTS AND CONCLUSION：The gray value and maximum torque value of regenerated osseous tissue at different time points in experimental group were significantly higher than those in the control group （P 〈 0.05）. Within 12 weeks of implantation, the bone formation rate and maturity of new bone tissue were higher in the experimental group compared with the control group, and no inflammatory cel infiltration occurred. At the 4th week after implantation, there was a large amount of inflammatory cel infiltration, and few inflammatory existed at the 8th week after implantation. These results demonstrate that compared with nanohydroxyapatite alone, the mixture of nanosilver and nanohydroxyapatite shows better antibacterial effect, biocompatibility and osteoinductive ability, which may accelerate osseointegration and promote osteogenesis.
出处
《中国组织工程研究》
CAS
北大核心
2015年第47期7649-7654,共6页
Chinese Journal of Tissue Engineering Research
基金
2011年度留学人员科技活动资助项目~~
