摘要
采用超声波辅助法提取红松种鳞多酚类物质以高效获取具有抗氧化活性的多酚。以多酚得率、DPPH·清除率为指标,通过单因素试验,研究超声时间、超声温度、超声功率对多酚得率及其抗氧化活性的影响。在单因素试验的基础上,以多酚得率为响应值,采用Box-Benhnken法设计三因素三水平的响应分析试验。结果表明:超声波辅助提取红松种鳞多酚的数学模型回归显著,最佳提取条件为:超声时间52 min、超声温度53℃、超声功率300 W。在此条件下,多酚得率可达27.80 mg/g,多酚得率与同温度水浴240 min得率相同,提取时间缩短2 h。此条件下提取的松多酚对DPPH·清除的IC50值为20.72μg/m L;对羟自由基清除率IC50值为20.69μg/m L;对超氧阴离子清除率的IC50值为259.71μg/m L,且具有较高的总还原能力。
We used the ultrasonic assisted method to extract the polyphenols from seed scales of Korean pine for the purpose of obtaining polyphenols with antioxidant activity efficiently. With polyphenols yield and DPPH radical scavenging rate as the indexes,we studied the effects of ultrasonic time,ultrasonic temperature,and ultrasonic power on the polyphenols yield and their antioxidant activity through single factor experiment. With polyphenols yield as the response value,and using the Box-Benhnken method,we designed the response analysis test with three factors and three levels. The results showed that the regression of mathematical model by ultrasonic assisted method for extracting polyphenols from seed scales of Korean pine is significant, and the optimal conditions are ultrasonic time 52 min, ultrasonic temperature 53 ℃,and ultrasonic power 300 W. Under these conditions,the polyphenols yield can reach27. 80 mg / g,comparable to that in water bath at the same temperature for 240 min,but extraction time is2 hours less. IC50 value of pine polyphenols is 20. 72 μg / mL for DPPH radical scavenging,20. 69 μg / mL for hydroxyl free radical clearance,and 259. 71 μg / mL in the ultra oxygen anion clearance. The pine polyphenols have high total reducing power.
出处
《北京林业大学学报》
CAS
CSCD
北大核心
2015年第11期128-135,共8页
Journal of Beijing Forestry University
基金
国家自然科学基金项目(C160131170510)
关键词
超声
多酚
提取
抗氧化
红松种鳞
ultrasonic
polyphenols
extraction
antioxidation
seed scales of Korean pine
