摘要
自噬是调节真核细胞生长、死亡和能量代谢的重要生物学机制。细胞自噬行使其生物学功能的前提是自噬流的活化。大量证据表明自噬流受损与多种慢性炎性疾病,特别是肿瘤、神经退行性疾病及组织纤维化的发病密切相关,目前对于自噬流的检测是自噬与其相关疾病研究领域的热点。由于自噬流是一个由多个步骤组成的动态过程,因此往往需要精细的实验才能准确判断自噬流状态。本文将介绍自噬流的基本概念并总结目前常规使用的自噬流检测方法。这些方法将有助于自噬生物学机制的研究,并为自噬相关药物筛选提供有效的解决方案。
Autophagy is a crucial biological process of eukaryotes, which is involved in cell growth, survival and energy metabolism, while the premise of the autophagy function is activated autophagic flux. It has been confirmed that impaired autophagic flux promotes pathogenesis of many chronic inflammatory diseases, especially cancer, neurodegenerative disease and tissue fibrosis, therefore the analysis of autophagic flux state is important for revealing autophagy function and the mechanism of autophagy related diseases. Given that autophagy is a dynamic process with multiple steps, it is very hard to observe the real state of autophagic flux. Summarized here is the novel concept and current approach to detect autophagic flux. This knowledge is crucial for the researching of the biological function of autophagy, and may provide some strategies for developing autophagy-related drug.
出处
《药学学报》
CAS
CSCD
北大核心
2016年第1期45-51,共7页
Acta Pharmaceutica Sinica
基金
国家自然科学基金资助项目(81503128)
中央级公益性科研院所基本科研业务费-创新药物与新技术专项资助项目(2014CX09)
关键词
自噬流
流式细胞术
串联荧光
长寿命蛋白
微管结合蛋白1A/1B-轻链3
autophagic flux
flow cytometry
tandem fluorescent
long lived protein
microtubule-associated protein 1A/1B-light chain 3
