摘要
从广东某化学试剂厂周边土壤中筛选、分离并纯化出一株能有效降解1,2,3,4-四氯苯(1,2,3,4-TeCB)的菌株,命名为L-1号菌.探索不同接种量(2%;3%;5%)菌株对25 mg·L-1的1,2,3,4-四氯苯模拟废水化学需氧量(COD)、氯离子释放浓度、1,2,3,4-四氯苯降解效果影响,并结合气相质谱联用仪(GC-MS)工作站分析菌株对1,2,3,4-TeCB降解产物.结果表明:经16S rDNA鉴定该菌株为枯草芽孢杆菌;菌株接种量为5%对1,2,3,4-TeCB降解效果最佳,在pH=7.0、温度30℃,摇床转速110 r·min-1条件下反应7d,COD降解率达62.71%;氯离子释放浓度4.98mg·L-1;四氯苯降解量达20.47 mg·L-1.通过对L-1号菌降解1,2,3,4-TeCB产物分析,发现L-1号菌对1,2,3,4-TeCB的降解是通过先开环后脱氯的途径实现的.
A 1,2,3,4-Tetrachlorobenzene（1,2,3,4-TeCB） degrading bacterial strain named L-1 was screened, isolated, and purified from surrounding soil of a chemical reagents factory in Guangdong Province. The degradation characteristics of 1,2,3,4-Tetrachlorobenzene（1,2,3,4-TeCB） in wastewater were studied for the chemical oxygen demand（COD） removal, chlorine release, and degradation rate of 1,2,3,4-TeCB under various strain inoculation（2%, 3% and 5%）. The degradation products of 1,2,3,4-TeCB by strain L-1 were analyzed by using gas chromatograph mass spectrometry（GC-MS）.The results showed strain L-1 wasBacillus subtilisas identified by partial length of 16 S RNA gene sequences.The optimal strain inoculation concentration was 5%. The degradation rate of COD was up to 62.71%, the chlorine released was 4.98 mg·L-1, and the degradation of 1,2,3,4-TeCB was up to 20.47mg·L-1 at the condition of pH 7.0, 30 ℃, with a rotating speed of 110 r·min-1in 7 days. Through the analysis of degradation products of 1,2,3,4-TeCB by GC-MS, it suggests that the degradation pathway of 1,2,3,4-TeCB was benzene ring cleavage firstly, followed by the chlorine removal from the parent compound.
出处
《环境科学学报》
CAS
CSCD
北大核心
2016年第1期106-111,共6页
Acta Scientiae Circumstantiae
基金
国家国际合作专项项目(No.2013DFG50150)
国家自然科学基金(No.40971251)~~
关键词
1
2
3
4-四氯苯
筛选
降解
氯离子
1
2
3
4-tetrachlorobenzene
isolation
degradation characteristics
chlorine
