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转铁蛋白修饰紫杉醇脂质体的制备及其抑瘤作用 被引量：8

Preparation and Tumor Inhibition Effect of Transferrin Modified Paclitaxel-loaded Liposome
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摘要 目的：制备转铁蛋白（TF）修饰紫杉醇（PTX）脂质体（TF-PTX-LP）,并研究其抑瘤作用。方法：采用薄膜分散法制备TF-PTX-LP并观察其形态;定性和定量考察肝癌Hep G2细胞对TF-LP和LP的摄取情况;检测PTX、PTX-LP和TF-PTX-LP分别处理Hep G2细胞24、48、72 h后细胞的增殖抑制率;以Hep G2细胞制备肿瘤球,考察生理盐水、PTX、PTX-LP和TF-PTX-LP分别处理0、1、2、4、5、6、7 d对肿瘤球体积的影响;复制Hep G2荷瘤裸鼠模型,考察生理盐水、PTX、PTX-LP和TF-PTX-LP（以PTX计8.5mg/kg）对荷瘤裸鼠移植瘤的抑制作用。结果：TF-PTX-LP均呈均一球形,粒径在100~120 nm。TF-LP处理后细胞的荧光强度强于LP（P〈0.01）。与PTX、PTX-LP比较,TF-PTX-LP处理后细胞的增殖抑制率更高（P〈0.01）。与生理盐水、PTX、PTX-LP比较,TF-PTX-LP处理后肿瘤球体积更小,对荷瘤裸鼠的抑瘤率更高,且6、7 d后抑瘤率差异有统计学意义（P〈0.01）。增殖抑制率和肿瘤球体积变化呈时间依赖性。结论：成功制得具有良好抑瘤作用的TF-PTX-LP。 OBJECTIVE：To prepare transferrin modified paclitaxel-loaded liposome（TF-PTX-LP）,and to study the tumor inhibition effect. METHODS：TF-PTX-LP was prepared by thin-film method,and morphology of TF-PTX-LP was observed. Qualitative and quantitative investigation were used to value the uptake efficiency of TF-LP and LP by Hep G2 cells. The proliferation inhibition rate of Hep G2 cells was investigated after treated with PTX,PTX-LP and TF-PTX-LP for 24,48 and 72 h. Tumor spheres were prepared by using Hep G2 cells. Effects of normal saline,PTX,PTX-LP and TF-PTX-LP on the volume of tumor spheres were investigated after 0,1,2,4,5,6 and 7 d treatment. Hep G2 tumor-bearing nude mice model was induced. Inhibitory effects of normal saline,PTX,PTX-LP and TF-PTX-LP（8.5 mg/kg by PTX）on transplantable tumor of tumor-bearing nude mice were investigated. RESULTS：TF-PTX-LP showed uniform spherical shape,with particle size of 100-120 nm. The fluorescence intensity of Hep G2 cells treated with TF-LP was stronger than that treated with LP（P〈0.01）. Compared with PTX and PTX-LP,TF-PTX-LP showed higher proliferation inhibition rate（P〈0.01）. Compared with normal saline,PTX and PTX-LP,tumor spheres were smaller in volume after treated with TF-PTX-LP,and inhibition rate of tumor was higher in tumor-bearing nude mice;there were statistical significance after treated for 6,7 d（P〈0.01）. The proliferation inhibition rate and tumor spheres volume changed in time-dependent manner. CONCLUSIONS：TF-PTX-LP which owns good tumor inhibition effect is prepared successfully.
作者 靳彩玲 赵树鹏 张敏 王颖 寇小格 路平
机构地区 新乡医学院第一附属医院肿瘤科 新乡医学院第一附属医院神经外科
出处 《中国药房》 CAS 北大核心 2016年第1期44-47,共4页 China Pharmacy
基金 河南省医学科技攻关计划项目(No.2011020091)
关键词 转铁蛋白 紫杉醇 脂质体 肝癌Hep G2细胞 裸鼠 增殖抑制率 抑瘤率 Transferrin Paclitaxel Liposome Lung cancer Hep G2 cell Nude mice Proliferation inhibition rate Tumor inhibition rate
分类号 R943 [医药卫生—药剂学]
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参考文献15

  • 1樊嘉,史颖弘.原发性肝癌研究：基础与临床的转化[J].中华肝胆外科杂志,2011,17(5):357-358. 被引量：24
  • 2Chen JG, Zhang SW. Liver cancer epidemic in China: past, present and future[J]. Seminars in Cancer Biology, 2011,21(1):59.
  • 3Jemal A, Bray F, Center MM, et al. Global cancer statis- tics[J]. CA CancerJClin, 2011, 61(2): 69.
  • 4Qin Y, Chen H, Zhang Q, et al. Liposome formulated with TAT-modified cholesterol for improving brain deliv- ery and therapeutic efficacy on brain glioma in animals [J]. Inter J Pharm, 2011,420 ( 2 ) : 304.
  • 5Zhang QY, Tang J, Fu L, et al. A pH-responsive a-heli- cal cell penetrating peptide-mediated liposomal delivery system[J]. Biomaterials, 2013,34 (32) : 7 980.
  • 6Yao Q, Chen H+ Yuan W, et al. Liposome formulated with TAT-modified cholesterol for enhancing the brain delivery [J]. Inter J Pharm, 201 I, 419 ( I ) : 85.
  • 7AI Soraj M, He L, Peynshaert K+ et al. siRNA and phar- macological inhibition of endocytic pathways to character- ize the differential role of macropinocytosis and the actincytoskeleton on cellular uptake of dextran and cationic cell penetrating peptides octaarginine (R8) and HIV-Tat [J]. J Control Release, 2012,161 ( 1 ) : 132.
  • 8Tang J, Zhang L, Liu Y, et al. Synergistic targeted deliv- ery of payload into tumor cells by dual-ligand liposomes co-modified with cholesterol anchored transferrin and TAT [J]. Inter J Pharm , 2013 , 454(1) : 31.
  • 9Gao H, Yang Z, Cao S, et al. Tumor cells and neo-vascu- lature dual targeting delivery for glioblastoma treatment [J]. Biomaterials, 2014,35 (7) : 2 374.
  • 10Yin Y, Wu X, Yang Z, et al. The potential efficacy of R8-modified paclitaxel-loaded liposomes on pulmonary arterial hypertension[J]. Pharm Res, 2013,30 (8) : 2 050.

二级参考文献18

  • 1Marincola FM. Translational medicine:a two-way road. J Transl Med, 2003,1:1.
  • 2Zhou XD, Tang ZY, Yang BH, et al. Hepatocellular carcino- ma: the role of screening. Asian Pac J Cancer Prey, 2000,1: 121-126.
  • 3Ji J, Shi J, Budhu A, et al. MicroRNA expression, survival, and response to interferon in liver cancer. N Engl J Med, 2009,361 : 1437-1447.
  • 4Qian YB, Zhang JB, Wu WZ, et al. P48 is a predictive marker for outcome of postoperative interferon-alpha treatment in pa- tients with hepatitis B virus injection-related hepatocellular carcinoma. Cancer, 2006,107 : 1562- 1569.
  • 5Llovet JM, Ricci S, Mazzaferro V, et al. Sorafenib in ad vanced hepatocellular carcinoma. N Engl J Med, 2008, 359: 378- 390.
  • 6Wilhelm SM, Carter C, Tang L, et al. BAY 43-9006 exhibits broad spectrum oral antitumor activity and targets the RAF/ MEK/ERK pathway and receptor tyrosine kinases involved in tumor progression and angiogenesis. Cancer Res, 2004, 64:7099-7109.
  • 7Tang ZY, Ye SL, Liu YK, et al. A decade's studies on metas tasis of hepatocellular carcinoma. J Cancer Res Clin Oncol, 2004,130 : 187-196.
  • 8Cheng BC, Zhou XP, Zhu Q, et al. Cobratoxin inhibits pain-evoked discharge of neurons in thalamic parafascicu- lar nucleus in rats: involvement of choIinergic and seroto- ner~ic systems[J]. Toxicon, 2009,54 (3) : 224.
  • 9Pang Z, Lu W, Gao H, et aI. Preparation and brain deliv- ery property of biodegradable polymersomes conjugated with OX26[J]. J Control Release, 2008,128 (2) : 120.
  • 10Chen S, Xu Y, Xu F, et al. Separation and determination of amino acids by micellar electrokinetic chromatographycoupling with novel multiphoton excited fluorescence de- tection[J]. J ChromatogrA ,2007,1 162(2) : 149.

共引文献23

同被引文献70

  • 1宿怀予,周劲.转铁蛋白与细胞穿膜肽共修饰脂质体的制备及其抗肿瘤效果[J].中国老年学杂志,2014,34(12):3363-3365. 被引量：2
  • 2Cho EJ,Sun B,Doh KO,et al.Intraperitoneal delivery of platinum with in-situ crosslinkable hyaluronic acid gel for local therapy of ovarian cancer[J].Biomaterials,2015,doi:10.1016/j.biomaterials.2014.10.039.
  • 3Wu X,He C,Wu Y,et al.Synergistic therapeutic effects of schiff's base cross-linked injectable hydrogels for local co-delivery of metformin and 5-fluorouracil in a mouse colon carcinoma model[J].Biomaterials,2016,doi:10.1016/j.biomaterials.2015.10.016.
  • 4Liu S,Wang X,Zhang Z,et al.Use of asymmetric multilayer polylactide nanofiber mats in controlled release of drugs and prevention of liver cancer recurrence after surgery in mice[J].Nanomedicine:NBM,2015,11(5):1 047.
  • 5Fakhari A,Subramony A.Engineered in-situ depot-forming hydrogels for intratumoral drug delivery[J].J Control Release,2015,220(Part A):465.
  • 6Lin Z,Gao W,Hu H,et al.Novel thermo-sensitive hydrogel system with paclitaxel nanocrystals:high drugloading,sustained drug release and extended local retention guaranteeing better efficacy and lower toxicity[J].JControl Release,2014,174(2):161.
  • 7Loo Y,Zhang S,Hauser CAE.From short peptides to nanofibers to macromolecular assemblies in biomedicine[J].Biotechnol Adv,2012,30(3):593.
  • 8Cormier AR,Pang X,Zimmerman MI,et al.Molecular structure of RADA16-I designer self-assembling peptide nanofibers[J].ACS Nano,2013,7(9):7 562.
  • 9Sundar S,Chen Y,Tong Y.Delivery of therapeutics and molecules using self-assembled peptides[J].Curr Med Chem,2014,21(22):2 469.
  • 10Tang F,Zhao X.Interaction between a self-assembling peptide and hydrophobic compounds[J].J Biomat SciPolym E,2010,21(5):677.

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中国药房
2016年 第1期

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