摘要
目的探讨多烯磷脂酰胆碱联合奥沙利铂对胃癌细胞增殖的影响。方法采用四甲基偶氮唑盐(MTT)比色法检测多烯磷脂酰胆碱、奥沙利铂及两药联合对胃癌SGC-7901细胞增殖的影响;流式细胞术检测各组细胞周期分布及凋亡情况;Western blotting检测各组凋亡相关蛋白细胞色素c、Bcl-2、Bax、caspase-9、caspase-3和细胞周期调控蛋白细胞因子D1、细胞因子E的表达水平。结果奥沙利铂显著抑制胃癌SGC-7901细胞增殖,且呈剂量和时间依赖性(P<0.05);多烯磷脂酰胆碱促进胃癌SGC-7901细胞增殖,其作用呈剂量依赖性(P<0.05);两药联合表现为协同作用,与奥沙利铂单药组比较,差异有统计学意义(P<0.05)。奥沙利铂使胃癌细胞增殖停滞在G_0/G_1期,并具有诱导胃癌细胞凋亡的作用;多烯磷脂酰胆碱可增强奥沙利铂诱导细胞凋亡及细胞周期停滞的作用,但这两种增强作用均与多烯磷脂酰胆碱的浓度无关(P>0.05)。Western blotting检测结果显示,多烯磷脂酰胆碱联合奥沙利铂上调细胞色素c的水平并激活其下游蛋白Bcl-2、Bax、caspase-9、caspase-3,下调细胞因子D1、细胞因子E的表达水平。结论多烯磷脂酰胆碱与奥沙利铂可抑制胃癌SGC-7901细胞的增殖和诱导细胞凋亡,将细胞阻滞于G_0/G_1期,两药联合可能会产生协同抗肿瘤的作用。
Objective To investigate the effect of polyenephosphatidylcholine（ PPC） combined with oxaliplatin（ L-OHP） on proliferation of gastric cancer cell lines. Methods After different concentrations of PPC and L-OHP treated on SGC7901 gastric cancer cells for 24,48,72 hours,MTT assay was used to examine the proliferation ability of each group. The MTT assay was used to calculate the proliferation ability of L-OHP at the concentration lower than the half-inhibitory concentration（ IC50） combined with different concentrations of PPC acting on SGC7901 gastric cancer cells for 48 hours. Flow cytometry was used to evaluate the apoptosis rate and analyze cell cycle of combined group. Western blotting was used to measure the expression levels of cytochrome c,Bcl-2,Bax,caspase-9,caspase-3,Cyclin D1 and Cyclin E. Results L-OHP could significantly inhibit the proliferation of SGC-7901 cells in a dose and time-dependent manner（ P〈0. 05）. The effect of PPC on the proliferation of SGC-7901 cells was dose-related（ P〈0. 05）,but not time-related（ P〉0. 05）. The anti-proliferation effect of PPC combined with L-OHP on SGC-7901 cells was synergistic,which had statistical significance compared with L-OHP alone（ P〈0. 05）. PPC greatly promoted cell apoptosis and G 0/ G 1 phase arrest induced by L-OHP. But the promotive effect was not related with the dose of PPC（ P〉0. 05）. PPC and L-OHP could upregulate the expression of cytochrome c and activate the following downstream protein including Bcl-2,Bax,caspase-9 and caspase-3,downregulate Cyclin D1 and Cyclin E expression. Conclusion PPC combined with L-OHP can inhibit the proliferation,induce the apoptosis of SGC-7901 cell lines,and block the cells at G 0/ G 1 phase,which shows a synergistic anti-tumor effect on gastric cancer cells.
出处
《临床肿瘤学杂志》
CAS
2015年第12期1074-1079,共6页
Chinese Clinical Oncology
