摘要
试验初步探讨了毛霉培养条件对其胞内代谢产物稳定性的影响。以单一菌株为研究对象,探讨了培养基、接种量、培养时间、培养温度、摇瓶速度等因素对菌株胞内代谢产物HPLC图谱的影响,分析各因素的影响大小,建立了规范化的培养流程:选用马铃薯制作马铃薯葡萄糖培养基,按每支18×180 mm试管装载30 m L进行分装,灭菌后接种2 m L的孢子悬液,在温度设为20℃的恒温摇床中以150 r/min摇瓶速度摇瓶培养4 d后取出。
The influence of culture conditions on Mucor intracellular metabolites stability was investigated in this paper. A Mucor strain was cultured in different medium, by different inoculation volume, for different culture time, at different culture temperature and different shaking speed, and then the cultures were detected on HPLC after extracting by 50% methanol solution. Through analysis of HPLC fingerprints, the standardized training process was established： potato dextrose was chose to be the medium, and the medium was packed to 18 x 180 mm test tube（30m L/ tube）. 2 m L of the spore suspension was inoculated in the medium after sterilization, and then the tube was placed in thermostatic shaker to culture for 4 d at 20 ℃ and 150 r/min.
出处
《西昌学院学报（自然科学版）》
2015年第4期25-28,共4页
Journal of Xichang University(Natural Science Edition)
关键词
毛霉
培养条件
胞内代谢产物
高效液相色谱
中药色谱指纹图谱
相似度评价系统
Mucor
intracellular metabolites
High performance liquid chromatography（HPLC）
Traditional Chinese Medicine（TCM）
chromatographic fingerprint similarity evaluation system
