摘要
目的 :探讨鼻粘膜上皮细胞应答局部组织缺氧 ,合成分泌诱发型一氧化氮合酶 (iNOS)的变化 ,以及地塞米松对此的影响及意义。方法 :将人鼻粘膜上皮细胞在常氧和缺氧状态下进行无血清原代细胞培养 ,并加入不同浓度的地塞米松共同孵育 ,采用流式细胞仪观察常氧和缺氧条件下上皮细胞动力周期的变化 ,采用原位杂交的方法检测iNOSmRNA的变化。结果 :缺氧条件下上皮细胞的动力周期延长 ;在缺氧 3h后iNOSmRNA水平开始升高 ,12h达高峰 ,2 4h后下降 ,4 8h几乎消失 ;加入地塞米松后 ,降低这种升高的水平。但 4 μg L以上的浓度 ,并不进一步降低被缺氧升高的iNOSmRNA水平。结论 :缺氧诱发鼻粘膜上皮细胞合成高水平的iNOSmR NA 。
Objective:To study the sythenesis of inducible isoform of nitric oxide synthase (iNOS) in human nasal mucosal epithelial cells (HNMECs) under hypoxia, and the role of dexamethasone on this process. Method:HNMECs were cultured without serum under normal condition and hypoxia for 3～48 hours, parts of them were cultured with dexamethasone. Cell cycle of epithelial cells was observed by flowcytometer. INOS mRNA was detected by in situ hybridization. Result:Cell cycle of epithelial cells became long under hypoxia. iNOS mRNA expression increased with time dependence after hypoxia for 3h compared with controls. The peak appeared after 12h hypoxia (P< 0.01 ). Increased iNOS mRNA expressions were reduced significantly by dexamethasone, but higher doses of dexamethasone (> 4.0 g/L) did not result in further suppression. Conclusion:Hypoxia can regulateexpression of iNOS mRNA significantly in HNMECs, and dexamethasone can restrain this process significantly.
出处
《临床耳鼻咽喉科杂志》
CAS
CSCD
北大核心
2002年第8期390-392,I005,共4页
Journal of Clinical Otorhinolaryngology
