摘要
考察清营汤对非那西丁和咪达唑仑在大鼠体内药代动力学的影响,进而探讨清营汤对CYP1A2和CYP3A1酶活性的影响。将大鼠随机分为空白对照组和清营汤组,清营汤组大鼠连续给药10 d,空白对照组大鼠给予相同体积生理盐水。第11 d,大鼠分别静脉注射非那西丁和咪达唑仑,高效液相色谱法分别测定非那西丁和咪达唑仑的血药浓度,DAS2.0软件计算药动学参数。结果清营汤组大鼠体内的非那西丁药代动力学参数较正常对照组出现显著差异,代谢速率加快;但是清营汤对咪达唑仑在大鼠的药代动力学特征没有显著影响。实验清营汤可能诱导大鼠体内的CYP1A2活性,但是对CYP3A1没有显著影响。
The influence of Qingying Decoction on the pharmacokinetics of phenacetin and midazolam in rats was studied, and the effect of Qingying Decoction on the activity of CYP1A2 and CYP3A1 enzymes was discussed. Rats were randomly divided into control group and Qingying decoction group. Each rat in Qingying decoction group was given Qingying decoction for 10 consecutive days and each rat in control group was given equal volume of saline. On day 11, rats were received a single dose of phenacetin and midazolam by intravenous injection, respectively. The concentrations of phenacetin and midazolam were determined by HPLC method. The pharmacokinetic parameters of the two chemicals were calculated with DAS2.0 software. Comparing with the control group, the pharmacokinetic parameters of phenacetin were significant changed in Qingying Decoction group. And the metabolic rate of phenacetin was speed up. But there was no significant difference in the pharmacokinetic parameters of midazolam. The results indicate that Qingying decoction can induce the activity of CYP1A2, but it has no effect on CYP3A1.
出处
《当代化工》
CAS
2015年第12期2752-2754,2758,共4页
Contemporary Chemical Industry
