摘要
采用高效液相色谱法(HPLC)建立大鼠血浆中的甲氨蝶呤(MTX)和特立氟胺(TEF)的检测方法,研究大鼠体内MTX和TEF的药代动力学。色谱柱为Aglient ZORBAX SB-C18柱(4.6 mm×250 mm,5μm);流动相:50 m M乙酸钠溶液—乙腈(体积比85:15);检测波长为300 nm;柱温为30℃;流速为1.5 m L/min。大鼠采用静脉注射给药,药动学参数采用DAS 2.0计算。该HPLC方法可用于大鼠血浆中MTX和TEF的同时测定,方法准确,快速,灵敏度高,重复性好,适用于大鼠体内MTX和TEF药物代谢动力学的研究。
A HPLC method was established for determination of plasma concentration of methotrexate and teriflunomide and study on the pharmacokinetics of methotrexate and teriflunomide in rats. The chromatographic conditions were as follows： a Aglient ZORBAX SB-C18 chromatographic column（250 mm × 4.6 mm, 5 μm） was used at 30 ℃. The mobile phase consisted of 50 m M sodium acetate solution-acetonitrile（85：15, v/v） and was pumped at a flow rate of 1.5 m L？min-1. The determination wavelength was 300 nm. Rats were received a single dose of drugs by intravenous injection. The pharmacokinetic parameters of methotrexate and teriflunomide were calculated with DAS 2.0 software. The HPLC method for determining methotrexate and teriflunomide concentration in rat plasma is accurate, rapid, simple, sensitive and repeatability and can be applied to pharmacokinetic studies of methotrexate and teriflunomide.
出处
《当代化工》
CAS
2015年第12期2916-2918,共3页
Contemporary Chemical Industry
