摘要
为了从深加工食品中提取高质量和数量的动物源性DNA片段,以优化后CTAB方法提取,用于PCR检测。根据鸭线粒体基因序列,设计合成检测鸭源性成分引物,进行PCR体系和反应条件的优化筛选,建立三重PCR方法;同时扩增出牛源性116bp、猪源性212bp和鸭源性322bp目的基因片段。建立的三重PCR方法特异地鉴定牛肉及其制品中掺假猪和鸭源性成分,对打击食品掺假伪造、审核食品标签、保障消费者知情权、维护消费者健康等方面有重要作用。
In order to get high quantity and quality animal-derived DNA extraction from processed food,the CTAB method was improved for the PCR test.According to duck mitochondrial gene sequences,primers were designed and synthesized for detecting PCR system,optimizing reaction conditions and screening,establishing a triple PCR method.The amplified lengths of fragments were 116 bp for bovine,212 bp for porcine,322 bp for duck,respectively.The triple PCR assay to distinguish pork and duck from beef was developed.Lower amounts of meat than declared on the label.These practices are of great importance in the fight of forged food,examining the food labels,protecting the right to know and the health concern of consumers.
出处
《食品与机械》
CSCD
北大核心
2015年第6期79-83,共5页
Food and Machinery
基金
甘肃省科技计划资助(编号:1309RTSA025
1009FTGA018
1306TTPA036)
