摘要
目的:建立基于实时荧光PCR技术的鸭源性成分的快速检测方法。方法:以鸭线粒体DNA序列为目的基因,设计并筛选了鸭源性特异性引物及探针,进行荧光定量PCR扩增,建立鸭源性成分检测方法。通过特异性、灵敏性、及盲样检测试验,对该体系进行验证。结果:该方法能够快速有效的检测鸭源性成分,具有较强的特异性及灵敏性,灵敏度约为0.01%(质量分数);通过市售盲样肉制品的检测,表明该体系可用于定性检测加工肉制品中的鸭源性成分。结论:该方法特异性强,灵敏度高,可用于对肉制品中鸭源性成分的掺假鉴别。
Objective： A rapid detection method based on real- time PCR was established to detect the components of duck origin. Methods： The duck mitochondrial DNA was taken as target gene. The duck origin specific primers and probe were designed and screened to do real- time PCR amplification.And the detection method of components of duck origin was established. The system was verified by specificity,sensitivity,and blind sample testing. Results： The method could fast and effectively detect the components of duck origin and had strong specificity and sensitivity. The sensitivity was about 0. 01%（ mass fraction）. Through market blind detection of meat product,the system could be used for qualitative detection of duck origin components in processed meat products. Conclusion： the method with strong specificity and high sensitivity could be used for identification of adulteration in meat duck origin components.
出处
《肉类工业》
2015年第1期51-53,共3页
Meat Industry
关键词
实时荧光
PCR
检测
鸭源性
肉制品
real-time fluorescence
PCR detection
duck origin components
meat products
