摘要
微生物及其活性产物在植物病害防治中的作用日益凸显,加快了人们对其的研究和开发利用。然而,传统纯培养技术获得的微生物不足环境总量的1%,99%以上的微生物尚不能培养,阻碍了人们对其结构和功能多样性的研究。近年,随着宏基因组技术的发展,大量非培养来源的活性物质被挖掘并应用于病害防治,为微生物基因资源及其活性产物的研究和应用开辟了新的途径。本文就宏基因组学研究过程:宏基因组DNA提取、文库构建和筛选、高通量测序和序列分析等进行了回顾,探讨了宏基因组学在生物防治中的重要作用,并对宏基因组学这门新型的学科在热带冬季瓜菜病害生物防治中的应用前景进行了展望。
Recently, microorganisms and their active metabolites have become more and more important in the biocontrol of plant diseases, which speeded up the research and utilization of active substances. However, the cultivable microorganisms constitute only a small fraction of the microbial diversity, less than 1% of the microorganisms in various environments are readily cultivable, more than 99% of the microbes which exist in the environment cannot be cultivated easily. So, it fetter the research of microbial structure and function diversity. In recent years, with the development of metagenomic technology, a large number of uncultured microbial active substances could be excavated and applied in plant diseases of prevention and treatment. It has opened up a new way for mining and utilization uncultured microbial genetic resources and secondary metabolites. In this paper, the metagenomic research process which include metagenomic DNA extraction, library construction and screening,high-throughput sequencing and sequence analysis, and the research achievements in recent years have be reviewed. The important role of metagenomics in biological control have be discussed. It has also be discussed whether the new subject of metagenomics own an application prospect of plant disease biocontrol to diseases of the tropical melon and vegetable in winter.
出处
《分子植物育种》
CAS
CSCD
北大核心
2015年第5期1169-1176,共8页
Molecular Plant Breeding
基金
国家自然科学基金(31160024
31360432)
十二五国家科技支撑计划(2012BAD19B06)
海南省农科院农业科技创新专项(CXZX201416)共同资助
关键词
宏基因组学
文库构建与筛选
冬季瓜菜
生物防治
Metagenomics
Construction and screening of library
Winter melons and vegetables
Biocontrol
