摘要
以海藻酸钠作为包埋剂、戊二醛作为交联剂和氯化钙作为填充剂对色氨酸合成酶基因工程菌进行固定化,同时探究3种物质和菌体负载量对固定化菌影响,响应面法优化色氨酸合成酶基因工程菌合成L-色氨酸。固定化色氨酸合成酶基因工程菌最优制备条件为:海藻酸钠28.92 g/L、戊二醛0.95 g/L、氯化钙19.82 g/L、菌体负载量25 g/L,底物L-丝氨酸质量浓度为10 g/L、固定化菌8 g,L-色氨酸产率为28.16%,固定化菌可连续使用15批次。
Using sodium alginate as embedding agent,glutaraldehyde as crosslinking agent and calcium chloride as filling agent immobilize the genetic engineering bacteria with tryptophan synthetase. The optimal immobilization conditions such as the concentration of sodium alginate,glutaraldehyde and calcium chloride and the bacteria load were investigated by response surface method. The optimized preparation conditions were as follows： 28. 92 g / L sodium alginate,0. 95 g / L glutaraldehyde,19. 82 g / L calcium chloride and 25 g / L bacteria load. The yield of L-tryptophan was 28. 16% under10 g / L L-serine and 8 g immobilized bacteria. The immobilized bacteria could be used continuously for15 times.
出处
《精细化工》
EI
CAS
CSCD
北大核心
2016年第1期24-30,共7页
Fine Chemicals
基金
安徽省优秀青年人才基金重点项目(2013SQRL086ZD)
宿州学院教授(博士)科研启动基金项目(2014jb06)
安徽省创新创业训练计划项目
安徽省质量工程项目(2015zjjh034
2015ckjh108)
宿州学院质量工程项目(szxyzyjh201402)
宿州区域发展协同创新中心开放课题~~
关键词
L-色氨酸
固定化
基因工程菌
生物工程
L-tryptophan
immobilization
genetic engineering bacteria
biological engineering
