摘要
【目的】研究野生中药资源血叶兰无菌播种技术,实现其快速繁殖,为保护与利用其种质资源提供参考。【方法】以血叶兰种子为外植体,探讨4种培养基MS,1/2MS、花宝1号3.0g/L、1/2花宝1号1.5 g/L对种子萌发的影响,在培养基中添加不同激素配比、不同有机复合物等,筛选适宜原球茎增殖与分化、壮苗与生根的培养基。【结果】在MS、1/2MS、花宝1号3.0 g/L、1/2花宝1号1.5 g/L 4种培养基中,最适于种子萌发的培养基为1/2花宝1号1.5 g/L,培养效果较差的是MS培养基;成熟血叶兰种子在1/2花宝1号1.5 g/L＋蔗糖25.0 g/L培养基中暗培养80 d左右形成原球茎团,萌发率为86.67%。在1/2花宝1号1.5 g/L＋6-BA 2.0 mg/L＋椰汁（CM）100 mL/L＋蔗糖25.0 g/L培养基中添加NAA 0.4~0.6 mg/L,原球茎团转接培养45 d左右形成原球茎和小芽的混合体,最高增殖倍数为6.11倍,增殖效果显著优于添加NAA 0.2和0.8mg/L的培养基（p〈0.05）。将培养获得的小芽转接至分别添加土豆汁10%、香蕉汁10%和CM 100 mL/L的花宝1号3.0g/L＋NAA 1.0 mg/L＋AC 1.0 g/L＋蔗糖25.0g/L培养基中,以添加香蕉汁10%的培养效果最佳,培养80 d后可形成完整植株,幼苗生根率为93.33%,株高7.19 cm。经温室大棚炼苗10d后,在泥炭土与1 cm火山石（3：1）的混合基质中,血叶兰试管苗移栽60 d后成活率达91.7%。【结论】以血叶兰种子为外植体,可建立获得较完善的血叶兰无菌播种育苗技术体系,该体系可用于血叶兰的种质资源保护、种苗生产及产业化开发等。
[Objective]The aseptic seeding and rapid propagation technology for wild Chinese medicine Ludisia discolor were studied in the present experiment in order to provide references for the protection and utilization of L.discolor germplasm resources.[Method]The seeds of L.discolor were used as explants to investigate the effects of four culture media（MS,1/2MS,Hyponex No.1 3.0 g/L,and 1/2 Hyponex No.1 1.5 g/L） on seed germination.The suitable media for multiplication and differentiation of protocorm,and rooting culture of plantlets were screened by using different hormone combinations（6-BA and NAA） and organic compounds（potato juice,banana juice and coconut juice）.[Result]Under the conditions of 4 culture media viz.,MS,1/2MS,Hyponex No.1 3.0 g/L and 1/2Hyponex No.1 1.5 g/L,the medium of 1/2Hyponex No.1 1.5 g/L was found to be suitable for seed germination of L.discolor,while MS medium had worse effects on seeds germination.After 80 days for dark culture,the seeds germinated well and developed into protocorms in medium of1/2 Hyponex No.1 1.5 g/L＋Sugar 25.0 g/L,and the germination rate was 86.67%.In culture medium of 1/2 Hyponex No.11.5 g/L＋6-BA 2.0 mg/L＋CM 100 mL/L＋Sugar 25.0 g/L with supplement of NAA 0.4-0.6 mg/L,a mixed body of protocorms and small buds were induced after culturing about 45 days,and the highest multiplication time was 6.11,which was better than those of media supplied with NAA 0.2 or 0.8 mg/L.By supplement of potato juice 10%,banana juice 10%and CM 100 mL/L in culture medium of Hyponex No.1 3.0 g/L＋NAA 1.0 mg/L＋AC 1.0 g/L＋Sugar 25.0 g/L,the small buds could grow into plantlets about 80 days later in medium with banana juice 10%,the rooting rate was 93.33%,and the plantlet height was 7.19 cm.After hardening-culture for 10 days in greenhouse and transplanting for 60 days,the survival rate of L.discolor plantlets reached 91.7%by using mixed matrix of peat soil and lava（3：1）.[Conclusion]A relatively perfect propagation technology system of L.discolor can be established by using aseptic seeds as explants,which could be used for the protection of L.discolor germplasm resources,seedling propagation and its industrial development.
出处
《南方农业学报》
CAS
CSCD
北大核心
2015年第11期1949-1952,共4页
Journal of Southern Agriculture
基金
海南省自然科学基金项目(312095)
关键词
血叶兰
无菌播种
快速繁殖
原球茎
有机复合物
L.discolor
aseptic seeding
rapid propagation
protocorm
organic compounds
