摘要
叶色突变体是研究植物光合作用机理的理想材料。该研究以小麦旗叶黄化转绿突变体LF2090及其野生型H_261为材料,对其主要农艺性状、光合色素含量、光合参数和叶绿体超微结构进行比较分析。结果显示:突变体在旗叶黄化期叶绿素b含量显著降低,叶绿体结构基本正常,光合速率无显著变化;在旗叶转绿期,突变体叶绿素b相对含量提高,但各色素含量均显著下降,叶绿体内基粒大部分消失,细胞间CO_2浓度及光合速率均显著下降。研究表明,叶绿素a与叶绿素b间的比例变化导致突变体旗叶叶片颜色发生由黄转绿的变化;突变体LF2090旗叶气孔部分关闭是该突变体光合速率降低和农艺性状较差的主要原因,同时色素含量降低导致叶绿体结构的改变也影响着旗叶的光合效率。
Leaf color mutant is an ideal material for analyzing plant photosynthesis mechanism.Agronomic traits,photosynthetic pigment content,photosynthetic parameters and chloroplast ultrastructure of wheat mutant LF2090 with yellow green-revertible flag leaf were investigated,while the wild type H261 was selected as control.The results showed that the content of chlorophyll-b in mutant had a significant reduction in the flag leaf yellowing period,the number of chloroplasts and grana were also reduced,but photosynthetic rate did not change significantly.While,the pigment content,CO2 concentration and photosynthetic rate of the mutant all dropped dramatically in the flag leaf green-revertible stage and most of grana in the chloroplast were disappeared,however,the relative content of chlorophyll-b was increased.The close of stomatas in the LF2090＇s flag leaf is the main reason to reduce the photosynthesis rate and the index of agronomic traits;the decrease of pigment content,which resulted in the abnormal chloroplast structure,also affects the photosynthetic rate.The change of proportion between chlorophyll-a and chlorophyll-b causes leaf color change from yellow to green.
出处
《西北植物学报》
CAS
CSCD
北大核心
2015年第12期2455-2461,共7页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家高技术研究发展计划(863计划)项目(2012AA101202)
陕西省科技统筹创新工程项目(2014KTZB02-01-01)
关键词
小麦
旗叶
黄化转绿
光合色素含量
光合参数
叶绿体超微结构
农艺性状
wheat
flag leaf
yellow green-revertible
the photosynthetic pigment content
photosynthetic parameters
the chloroplast ultrastructure
agronomic traits
