摘要
多酚是烟草重要的次生代谢物和香气物质前体。本研究建立了一种烟叶中多酚的超高效液相色谱-串联质谱定量分析方法,方法相对于现行行业标准(YC/T 202-2006,仅3个多酚指标)增加了15个新的分析指标。比较了不同比例的甲醇-水体系以及甲醇-水-氯仿体系(5∶2∶2,V/V)对烟叶中多酚的提取效果,发现甲醇-水-氯仿体系(5∶2∶2,V/V)的效果最佳。方法对所有被测多酚的线性相关系数(R^2)均在0.99以上,检出限在1~150μg/L之间,定量限在6~350μg/L之间,日内重复性在2.4%~6.5%之间,日间重复性在4.5%~10.1%之间,回收率在89.5%~103.7%之间,符合定量分析的要求。应用本方法检测经不同温度处理的烟草的多酚含量,发现在给定的实验条件下,多酚对环境温度变化的应答可分为随温度升高而降低、随温度升高而升高、无论温度升高和降低,含量均降低、以及与温度变化无显著相关4种模式。
Polyphenols are very important secondary metabolites for tobacco plants. They are also considered as the important flavor precursors for cigarette industry. A liquid chromatography-tandem mass spectrometry（ LC-MS /MS）-based method was established for the simultaneous determination of 18 polyphenols in tobacco leaf. The developed method can be used to determine 15 more polyphenols compared to the standard method for industry（ YC / T 202-2006）. Different kinds of extraction solution were compared and a solution of methanol-water-chloroform（ 5∶ 2∶ 2,V / V） was selected for the extraction because of its highest yield. Method validation was performed and the result showed that all the 18 polyphenols have their linear correlation coefficients（ R^2） more than 0. 99. The low limit of determination and the low limit of quantitation were in the range of 1- 150 μg / L and 6- 350 μg / L,respectively. Intra-day and Inter-day reproducibility were in the range of 2. 4%- 6. 5% and 4. 5%- 10. 1%,respectively. Recoveries were in the range of 89. 5%- 103.7%. The established method was then successfully used to analyze polyphenols of tobacco leaves planted under different environment temperatures. The polyphenols can be classified in four groups based on their response to the temperature changing： the concentration increase with the increasing environment temperature,the concentration decrease with the increasing environment temperature,the concentration decrease with the environment temperature changes from the normal set point, and the concentration does not change significantly with the changing environment temperature.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2015年第12期1913-1919,共7页
Chinese Journal of Analytical Chemistry
基金
云南省烟草专卖局基金资助项目(No.2014YN11)
关键词
烟草
多酚
超高效液相色谱
串联质谱
Tobacco
Polyphenols
Ultra performance liquid chromatography
Tandem mass spectrometry
