摘要
目的探索低频电刺激对中国树鼩死亡后脏器中激素水平的影响。方法给予中国树鼩低频电刺激,分别于死亡后0、3、6、12、18、24、36 h和72 h取甲状腺、肝、脾,用放射免疫法测定内皮素(endothelin,ET)、心钠素(atrial natriuretic factor,ANF)、血栓素(thromboxane,TX)水平;剥取死后0 h中国树鼩中脑腹侧背盖区(ventral tegmental area,VTA)检测c-fos表达。结果电刺激后,中国树鼩尸体脏器中内皮素、心钠素、血栓素水平比对照组显著升高,其水平随死亡后时间的延长而降低;VTA c-fos表达明显增强。结论低频电刺激能引起中国树鼩脏器中激素的合成、释放和脑组织c-fos表达。
Objective To study the effect of low frequency electrical stimulation on hormone levels in organs of Chinese tree shrews after death. Methods Giving Chinese tree shrews low frequency electrical stimulation. At 0 h,3 h,6h,12 h,18 h,24 h,36 h and 72 h after death,the thyroid,liver,spleen were taken,and the levels of endothelin（ ET）,atrial natriuretic factor（ ANF）,thromboxane（ TX） were determined by RIA method. At 0 h after death,midbrain ventral tegmental area（ VTA） of Chinese tree shrews was taken to detect the c-fos expression. Results After electrical stimulation,ET,ANF,TX levels in the cadaver organs and VTA c-fos expression of Chinese tree shrews were significantly increased than in the control group. The contents were decreasing with the time after death. Conclusions Low frequency electrical stimulation can induce the synthesis and release of hormones in organs and c-fos expression in brain tissue of Chinese tree shrews.
出处
《中国实验动物学报》
CAS
CSCD
北大核心
2015年第6期578-581,共4页
Acta Laboratorium Animalis Scientia Sinica
基金
国家自然科学基金项目(编号:31060283)
国家社会科学基金项目(编号:07BSH054)
甘肃省基础研究创新群体项目(编号:145RJIA333)
甘肃省科技支撑计划-社会发展类项目(编号:1304FKCA082)
甘肃政法学院重点项目(编号:GZF2014XZDLW18)
关键词
中国树鼩
尸体
脏器
激素
C-FOS表达
Chinese tree shrews
Cadaver
Visceral organs
Hormone
C-fos expression
Electrical stimulation
