摘要
为了提高黄淮海麦区小麦育种材料的赤霉病抗性,采用分子标记辅助选择的方法,将来自望水白的4个抗赤霉病主效QTL 3B-QTL、4B-QTL、5A-QTL和6B-QTL导入不同的感病背景中,在后代BC1F3和BC1F4株系中评价它们的抗病效应和农艺性状回复情况。结果表明:(1)导入4个抗病QTL株系的平均病小穗率和病粒率分别为12.2%和6.3%,而受体亲本则分别达到59.1%和44.2%,抗病性显著提高;(2)病小穗数和病粒率与穗长及株高极显著负相关,但与可育小穗数、百粒重、旗叶长和旗叶宽等农艺性状指标没有显著相关性。因此,通过导入抗病主效QTL可以显著改善感病材料的抗性,为进一步选育高产抗病品种提供基础材料。不良农艺性状的紧密连锁阻碍着抗赤霉病主效QTL的高效利用,需要通过继续回交或与其他品种杂交来打破这种遗传连锁关系。
Based on molecular marker-assisted selection,four major QTL genes were transferred into six different susceptible genetic backgrounds to improve wheat scab tolerance in the Huang-huai-hai area derived from a common wheat landrace wangshuibai chromosome 3B,6B,4B,and 5A. Their resistance and agronomic traits were evaluated from field trail at BC1F3 and BC1F4progeny lines for two consecutive years. The results showed that the average percentage of diseased spikelet and fuasrium diseased kernel of the lines carried four QTLs was 12. 2% and6. 3% while those of the susceptible recipient parents up to 59. 1% and 44. 2%,respectively. The wheat lines performed more significantly than their recipient parents in the disease resistance,and that the plant height and spike length showed apparently negative correlation with percentage of diseased spikelet and fuasrium diseased kernel,but there was no significant differences between lines and recipient parents in flag leaf width,fertile spikelet number,grain number and hundred grain weight. The resistance to wheat scab would be enhanced by pyramided major QTLs in susceptible germplasm. Therefore,the lines with four QTLs could be applied for further breeding practice,but backcrossing or cross with other varieties more times could be helpful in breaking the close linkage of major QTLs to the adverse agronomic traits.
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2016年第1期132-139,共8页
Journal of Plant Genetic Resources
基金
安徽省教育厅自然科学重点项目(KJ2015A221)
安徽科技学院重点学科项目(AKZDXK2015A03)
关键词
小麦
赤霉病抗性
基因聚合
农艺性状
wheat
resistance to scab
QTL pyramiding
agronomic traits
