摘要
目的:观察脑缺血再灌注损伤后左卡尼汀对caspase-3表达的影响。方法:40只Wistar雄性健康大鼠,随机分为A组(假手术组)、B组(对照组)、C组(左卡尼汀100 mg/kg治疗组)和D组(左卡尼汀200 mg/kg治疗组)。线栓法制造大鼠大脑中动脉缺血再灌注模型,观察左卡尼汀对大鼠神经功能症状、组织形态学改变以及对caspase-3表达的影响。结果:与B组比较,左卡尼汀各治疗组能显著改善大鼠神经功能缺失症状,减少脑组织的梗死缺血损伤,降低caspase-3的表达(P<0.01)。结论:左卡尼汀通过抑制脑缺血再灌注损伤后脑组织中caspase-3的表达起到保护神经细胞的作用。
Objective： To observe the effect of levocarnitine on the expression of caspase- 3 in the rat model of focal cerebral ischemia- reperfusion. Methods： Forty healthy male Wistar rats were divided into four groups randomly： sham operation group（ A group）,saline- controled group（ B group）,treated with 100 mg / kg of levocarnitine group（ C group） and treated with200 mg / kg of levocarnitine group（ D group）. The focal cerebral I / R rats models were made by the MCAO. The neurological deficit score,histological changes of brain and the expression of caspase- 3 in cortex and hippocampus were detected. Results：The neurological deficit score,histological changes and the expression of caspase- 3 in cortex and hippocampus were significantly decreased（ P〈0. 01） in levocarnitine group compared with B group. Conclusion： The results indicated that Levocarnitine has neuro protective effect to decrease the expression of caspase- 3 protein against cerebral I / R induced injury in rat brain.
出处
《临床医药实践》
2016年第1期10-12,共3页
Proceeding of Clinical Medicine
基金
山西省科技攻关项目(项目编号:20100311098-1)
