摘要
目的基于气体信号分子途径,分析阿魏酸(ferulic acid)对大鼠离体胸主动脉舒缩功能的影响。方法采用大鼠离体胸主动脉环,加入3种气体信号分子的抑制剂,即一氧化氮合酶的抑制剂NG-硝基-L-精氨酸甲酯(L-NAME)、血红素加氧酶-1(HO-1)抑制剂锌卟啉(Zn PP)、胱硫醚-!裂解酶抑制剂炔丙基甘氨酸(PAG),进行体外血管舒张实验。结果加入3种气体信号分子抑制剂均能显著抑制阿魏酸的舒张血管作用(与空白组相比分别为P<0.01,P<0.01,和P<0.05),且不同浓度的阿魏酸均使Ca Cl2量效曲线右移(P<0.01),且可显著性降低无钙高钾K-H液中去甲肾上腺素NE引起的收缩(P<0.01)。结论阿魏酸的舒血管作用由NO、CO和H2S 3种气体信号分子通路介导,并通过血管平滑肌上的电压依赖性钙通道和受体依赖性钙通道发挥作用。
AIM To investigate the vasorelaxant effect of ferulic acid on thoracic aortic rings isolated from rats. METHODS Three gas signal molecule inhibitors,including nitric oxide synthase inhibitor NG-nitro-L-arginine methyl ester（ L-NAME）,heme oxygenase 1（ HO-1） inhibitor zinc-protoporphyrin（ Zn PP） and urinary sulfur ether-lyase inhibitor DL-propargylglycine（ PAG）,were incubated with rats ＇ thoracic aortic rings,and then recorded in vitro vasorelaxant information. RESULTS Three gas signal molecule inhibitors could markedly inhibit vasorelaxant effect of ferulic acid compared with the control group（ P〈0. 01,P〈0. 01 and P〈0. 05）. Ferulic acid made the dose-effect curve of Ca Cl2 right movement（ P〈0. 01） and noticeably inhibited extracellular Ca2 ＋-induced contraction in high-K＋and NE pre-challenged rings. CONCLUSION The vasorelaxant effect of fulic acid is related to the presence of three gas signal inhibitors and with the help of voltage dependent calcium channel and receptor dependent calcium channel on vascular smooth muscle.
出处
《中成药》
CAS
CSCD
北大核心
2016年第1期6-12,共7页
Chinese Traditional Patent Medicine
