摘要
目的:观察覆盆子不同提取部位对东莨菪碱、亚硝酸钠及40%乙醇造成的小鼠记忆障碍模型的影响,探索覆盆子对这3种记忆障碍模型的有效部位,为进一步研究覆盆子对学习记忆改善作用的机制提供依据。方法:昆明种小鼠130只,随机分成13组,每组10只。即正常组,模型组,阳性组,覆盆子全药高剂量和低剂量组,三氯甲烷部位高剂量和低剂量组,正丁醇部位高剂量和低剂量组,乙酸乙酯部位高剂量和低剂量组,水提部位高剂量和低剂量组。给药组小鼠分别以高、低剂量(24,12 g·kg^(-1))ig覆盆子全药、三氯甲烷部位、正丁醇部位、乙酸乙酯部位、水提部位;阳性药组给银杏叶制剂量为7.6 mg·kg^(-1),正常组和模型组给予等量的蒸馏水,连续12 d。7 d后除正常组外,东莨菪碱所致模型测试前30 min ip 1 mg·kg^(-1),亚硝酸钠所致模型训练后ip 120 mg·kg^(-1),40%乙醇所致模型测试前30 min ig 0.1 m L·kg^(-1),检测小鼠在Morris水迷宫的定位航行能力和空间探索能力。结果:与正常组比较,3种记忆障碍逃避潜伏期明显延长,穿越平台次数明显缩短(P<0.05);与模型组比较,覆盆子全药、三氯甲烷部位、正丁醇部位、乙酸乙酯部位、水提部位均可缩短3种记忆障碍逃避潜伏期,增加其穿越平台次数(P<0.05)。结论:覆盆子水提部位高剂量组对东莨菪碱、亚硝酸钠造成的小鼠记忆障碍模型改善效果最优;覆盆子全药高剂量组对40%乙醇造成的记忆障碍小鼠模型改善效果最优。
Objective： To investigate the effect of Rubi Fructus＇ s different extract parts on the memory disorders induced by scopolamine,Sodium nitrite and 40% Ethanol in rats,explore the effective extract parts of Rubi Fructus for the 3 memory disorder models,and provide the experimental evidence for research of mechanism of Rubi Fructus in improving the memory ability. Method： Intragastric 130 Kunming mice were randomly divided into 13 groups,n = 10 in each group,including blank group,model group,positive group,Rubi Fructus whole prescription high dose group and low dose group,chloroform extract high dose group and low dose group,n-butyl alcohol extract high dose group and low dose group,ethyl acetate extract high dose group and low dose group,aqueous extract high dose group and low dose group. Administration groups respectively received ig high dose and low dose（24,12 g·kg^-1） Rubi Fructus whole prescription,chloroform extract,n-butyl alcohol extract,ethyl acetate extract,and aqueous extract. The positive group received ginkgo leaves preparation 7. 6 mg·kg^-1. The blank group and the model group received same-volume-gavage of saline,continuous for 12 days. After 7 days,all mice except those in the blank group were intraperitoneally injected with corresponding 1 mg·kg^-1 dose 30 min before test for the scopolamine-induced models,intraperitoneally injected with corresponding 120 mg ·kg^-1 after training for the sodium nitrite-induced models,and received intragastric administration of 0. 1 m L·kg^-1 30 min before test for 40% ethanol-induced models,to explore the navigation ability and space exploration ability in the Morris water maze. Result： Compared with the normal group,escape latency of three memory disorders were significantly prolonged,and the number of times for passing the platform was significantly reduced（P 〈0. 05）. Compared with the model group,Rubi Fructus whole prescription,chloroform extract,ethyl acetate extract,n-butanol extract,and water extract can shorten the escape latency of three memory disorders and enhance the number of times for passing the platform（P 〈0. 05）. Conclusion： Rubi Fructus ＇ s water extract high dose group has the best effect for improving the memory disorders induced by scopolamine and sodium nitrite. Rubi Fructus whole prescription high dose group has the best effect for improving memory disorders induced by 40% ethanol in mice.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2016年第2期142-147,共6页
Chinese Journal of Experimental Traditional Medical Formulae
基金
江西省自然科学基金项目(20133BAB20002)
江西省卫生厅中医药科研计划项目(2013A010)
江西省高等学校大学生创新创业计划项目(201310412005)
关键词
覆盆子
学习记忆
东莨菪碱
亚硝酸钠
40%乙醇
Rubi Fructus
learning and memory
scopolamine
sodium nitrite
40% ethanol
