摘要
药用植物绞股蓝内生真菌JY25胞外多糖具有抗癌、抗氧化等多种活性作用,对其发酵条件进行优化,以期达到高产胞外多糖的效果。以胞外多糖产量为检验指标,采用单因素和正交试验对培养时间、接种量、碳源、氮源进行优化。单因素试验结果表明,绞股蓝内生真菌JY25产胞外多糖的最佳培养周期为8 d,最佳接种量为7%,最佳碳源为葡萄糖,最佳氮源为酵母粉;正交试验优化后发现,产胞外多糖的最佳发酵条件为培养时间8 d、接种量5%、葡萄糖添加量40 g/L、酵母粉添加量5 g/L,在此条件下胞外多糖的产量达到271.40 mg/L。
Extracellular polysaccharide produced by endophytic fungi JY25 from the medicinal plants Gynostemma pentaphyllum had anti-cancer,anti-oxidation and other bioactive effects. The fermentation conditions were optimized to obtain the highest EPS. The incubation time,inoculum dose,carbon sources and nitrogen sources were optimized by single factor test and the orthogonal test with the yield of extracellular polysaccharide as the test aim. The optimized results of single factor test showed that the optimum culture cycle was 8 d,the optimum inoculum dose was 7%,the optimum carbon source was glucose,and the optimum nitrogen source was yeast powder. The optimized results of orthogonal test indicated that the optimum culture cycle was 8 d,the optimum inoculum dose was 5%,the optimum application rate of glucose was40 g / L,and the optimum application rate of yeast powder was 5 g / L,under the above conditions the yield of extracellular polysaccharide reached 271. 40 mg / L.
出处
《河南农业科学》
CSCD
北大核心
2016年第1期143-146,共4页
Journal of Henan Agricultural Sciences
基金
河南省教育厅自然科学项目科学技术重点研究项目(15A180013)
关键词
绞股蓝
内生真菌
胞外多糖
发酵条件
优化
Gynostemma pentaphyllum
endophytic fungi
extracellular polysaccharide
fermentation conditions
optimization
