摘要
目的探讨噪声致感音神经性聋豚鼠模型听觉电生理及内耳形态学的改变。方法 2014年1月—2015年1月承德医学院选取豚鼠24只按随机数字表法分为对照组和实验组,每组12只。实验组豚鼠给予白噪声暴露1周,暴露声压级(A计权)为110 dB,每日暴露1次,每次1.5 h,连续暴露1周;对照组豚鼠安静环境中饲养,未给予噪声暴露。分别测试噪声暴露前1天,噪声暴露后1、2及4周听性脑干反应(ABR)阈值及80 dB nHL下Ⅰ波潜伏期,并通过扫描电镜及光镜观察噪声暴露后4周豚鼠耳蜗毛细胞的形态变化。结果与对照组比较,实验组在噪声暴露后1、2、4周体质量下降(P<0.01),且ABR阈值均不同程度上升,Ⅰ波潜伏期也逐渐延长,差异具有统计学意义(P均<0.01)。扫描电镜显示,对照组豚鼠耳蜗内、外毛细胞形态正常,排列整齐,界限清楚;实验组豚鼠耳蜗内、外毛细胞均出现紊乱、融合及缺失。光镜显示:对照组耳蜗外毛细胞未见明显缺失,实验组耳蜗外毛细胞数有显著缺失,2组之间比较差异有统计学意义(P<0.05)。结论噪声致感音神经性聋后豚鼠听觉系统受到损害,毛细胞出现缺失、坏死,考虑永久性阈移(PTS)的产生可能与耳蜗外毛细胞异常及缺失有关。
Objective To investigate the changes of auditory electrophysiology and morphology of the inner ear in guinea pig model of noise induced hearing loss.Methods From Jan 2014 to Jan 2015,in Chengde Medical College,twentyfour guinea pigs were randomly divided into control and experimental groups with each group of 12 guinea pigs.The experimental group＇s guinea pigs exposed to white noise for one week,the exposure SPL（A-weighted） was 110 dB,exposed once a day,every 1.5 h,continuous exposure to one week;the control group of guinea pigs bred in a quiet environment,did not give any noise exposure.Noise exposure were tested before and after noise exposure at 1,2 and 4 weeks＇ auditory brainstem response（ABR） thresholds and 80 dB nHL I wave latency,and four weeks later,under electron microscopy and light microscopy to observe cochlea hair cell morphology changes after noise exposure.Results Compared to the control group,the experimental group after noise exposure for 1,2,4 week,the body weight were decreased（P〈0.01）,and the ABR thresholds were elevated,and the latency of wave I was gradually extended,the difference were statistically significant（P〈0.01）.The electron microscopy scan showed that the morphology of the inner and outer hair cells of the control group was normal,the arrangement of the cells was clear,the boundary was clear,light microscopy showed that the control group hair cells revealed no obvious loss.The experimental group outer hair cell number had a significant loss,the differences between the 2 groups showed statistical significance（P〈0.05）.Conclusion The auditory system of guinea pigs after noise induced hearing loss is damaged,and the hair cells appear to be absent and necrosis,and the generation of permanent threshold shift（PTS） may be related to the abnormal cilia of hair cells.
出处
《疑难病杂志》
CAS
2016年第1期58-60,64,F0003,共5页
Chinese Journal of Difficult and Complicated Cases
关键词
感音神经性聋
听觉电生理
耳蜗
毛细胞
豚鼠
Sensorineural hearing loss
Auditory electrophysiology
Cochlea
Hair cells
Guinea pig
